Notes -''^7 



As a culture medium, saline egg-albumen consisting of 5 per cent. 

 NaCl solution 100 c.c, white of new-laid egg 15 c.c. was used. 



Film preparations were made by floating cover-slips on the surface 

 or by placing them at the bottom of the cultures. The films thus 

 obtained were usually fixed in Maier's solution and stained with iron- 

 haematoxyUn. 



It is not the purpose of this paper to go into details concerning 

 the different species of protozoa found, but especially to put on record 

 the remarkable fact of the survival of protozoa for practically 50 years 

 under conditions precluding the possibility of trophic existence. 



The following protozoa have been identified. The list does not 

 include all the forms encountered, for there are a few which I have 

 not been able to classify satisfactorily yet. 



Aynoebae belonging to the Umax group were obtained from all the 

 soils which yielded protozoa. I have made observations on the mode 

 of nuclear division in three of these with the result that all three appear 

 to be new species. One of them seems to be very closely related to 

 Amoeba glebae^ Dobell. Flagellates: Monas termo, Cercomonas crassi- 

 cauda, Cercomonas longicauda, Bodo (ProwazeUa) saltans, Bodo ovatus^. 

 and Tetramitus spiralis, nov. spec. 



In addition to the above I have obtained a rather remarkable 

 organism which undoubtedly belongs to the genus Spironema Klebs^. 

 Klebs obtained his examples from ditch-water on only two or three 

 occasions and considered that they showed affinities with cihates 

 and flagellates, forming perhaps a sort of connecting link between 

 these two groups. He did not, however, find out anythmg about 



the nucleus. 



I have succeeded in making out the nucleus which, though pecuhar, 

 exhibits flagellate rather than ciliate affinities. 



Historical. 



It may be of interest, in view of the great length of time which 

 the resting cysts of these protozoa have retained their vitality, to state 

 briefly the previous records of similar phenomena. 



According to Butschh^, Meunier (1865) saw Colpoda emerge from 

 cysts dried for fourteen months. Balbiani (1881) kept a shde with 



1 "Cytological Studies on three species of Amoeba, etc." Archiv /. Protislen. Bd. 34 



(1914), p. 139. 



2 FUigdlatenstudien I. Zeitschr. f. iviss. Zool. lv (1892), p. 350. 



3 Bionn's Thierreichs 1, Abth. iii, p. 1663. 



