E. M. Doidgk 27 



Loftier* s blood serum. Streak cultures on Loffler's blood serum at 

 37°C. at the end of 18 hours show a shining, yellowish growth along the 

 needle track ; after 48 hours this becomes deep chrome yellow, forming 

 a streak about 2 mm. broad with smooth edges ; it is shining and not 

 raised above the surface of the medium. No liquefaction is observed 

 in tubes kept under observation for several weeks. 



Starch Jelly. There was no growth in starch jelly made with 

 Uschinsky's solution even in tubes kept under observation for several 

 weeks. 



Potato. The first evidence of growth on potato cylinders is a slight, 

 shining yellowish growth along the needle track; it then spreads over 

 the lower, moist portion of the cylinder, but on the upper half there 

 is no further growth. The colour is buff yellow (IV). There is no 

 greying or other discolouration of the medium. 



Mango. On pieces of mango sterilised by steaming in Roux tubes 

 the organism forms a cream-coloured, spreading growth covering the 

 surface of the medium. The mango was not discoloured. 



Cocoanut. This is a good medium for chromogenesis, but a plentiful 

 growth is not obtained unless the medium is moist. At 25°C. and 30°C. 

 growth was just visible in 24 hours, first appearing as a shining streak 

 along the needle track ; in 48 hours a glistening, cream-coloured growth 

 covered all the lower part of the cylinder. This was the wettest part 

 of the medium, as the pieces of cocoanut were steamed in Roux tubes 

 whose bulb was filled with water, or in ordinary test tubes resting 

 on a wad of wet cotton wool. In three days the colour was slightly 

 deepened, the water in the bulb of the Roux tubes clouded, and in 

 tubes where the cocoanut was resting on cotton wool, the upper layers 

 of this substratum were slightly yellow. The colour deepened and 

 became buff yellow (IV. 19 YOI — Y, d). In the Roux tubes, a yellow 

 sediment formed at the bottom of the bulb, and a ring on the glass 

 just above the liquid. As soon as the medium began to dry up growth 

 ceased, and no further changes took place in the appearance of the 

 culture. 



Beet. Tubes containing cylinders of beetroot were prepared in a 

 similar way to those containing cocoanut. The organism grows very 

 vigorously on beet; the whole of the cylinder became covered with a 

 shining, wet-looking growth, the upper part having a granular appear- 

 ance, and the lower portion being wrinkled. In the Roux tubes a 

 heavy pellicle was formed on the surface of the water in the bulb, and 

 in the straight tubes where the cylinder was resting on wet cotton wool 



