E. M. Doidge 29 



Usckinskifs solution. The bacillus would not grow in this solution. 



Cohrfs solution. A slight clouding took place after three days. No 

 ring or pellicle formed, and there was no sediment. 



Beerwort. The tubes were kept under observation for a considerable 

 length of time, but they remained clear. 



Potato broth. Abundant growth took place in Appel's potato broth. 

 A ring and pellicle were formed. 



Cabbage broth. In cabbage broth the organism caused very heavy 

 clouding; there was not much pellicle, but a fairly wide ring formed, 

 and there was about | cm. of sediment at the bottom of the tubes. 

 The upper part of the ring was yellow, but the lower part, which was in 

 contact with the broth, was light salmon orange (11). The sediment 

 was the same peculiar colour. 



Beet juice. Very heavy clouding took place in this medium, the 

 ring and pellicle were very well developed in colour, the growth was 

 similar to that formed on solid beet. 



Solution N. A fair amount of growth was observed in Marshall 

 Ward's Solution N, the medium clouded, but there was no formation of 

 ring or pellicle. 



Physical and Biochemical Features. 



In this section of the work, unless otherwise stated, the methods 

 used are those outlined by Eyre (8). Each of the tests was repeated 

 several times, and in no case were the results contradictory, although 

 they varied slightly with the vigour of the culture used. Even where 

 not specially mentioned, each experiment was checked by the use of 

 controls. 



Enzyme production. The organism is capable of dissolving the 

 middle lamella of cells in the tissues of the host plant, but it has 

 apparently no action on cellulose. 



A series of experiments conducted to test for the presence of various 

 enzymes in cultures of the bacillus, for the most part led to negative 

 results. A brief outline of these experiments follows. 



Diastolic enzymes. Four tubes of nutrient broth of optimum reaction 

 were inoculated and incubated for five days at 30° C. The cultures 

 were removed from the incubator on the fifth day, and mixed with equal 

 quantities of starch paste containing 2 % of thymol ; they were then 

 placed in the incubator at 37° C. for six hours, and at the end of that 

 time were tested for sugar with Fehling's solution. There was no 

 reaction for sugar. 



