72 



Pink Disease of Plantation Rubber 



of the attempts to start cultures from small pieces of mycelium were 

 unsuccessful. 



The cultures on wood blocks obtained in the first place from Necator 

 spores develop quickly. The mycelium grows profusely and remains 

 white for seven to ten days. It spreads over the block and into the 



vU'jOlt 



Ccttes wool 





V'O 







Fig. 11. Culture tube: wood block covered with mycelium which has grown into water 

 reservoir covering the water witli a film of hyphae on which Necator-like masses 

 have formed. Large Necalor-\ike masses at top of wood block. 



cotton wool placed at the base of the tube. Growing in the cotton 

 wool the mycelium begins to turn a pale pink colour which gradually 

 spreads over the whole culture. Subsequently the mycelium in the 

 cotton wool becomes a bright rose colour. The mycelium passes from 



