278 "Wilt" or "Crown-Mot" Disease of Carnations 



a small piece of the fungus on agar and pressing it past the bend of 

 the fermentation tubes; another piece of growth was placed on the 

 surface of the liquid in the open end. The pieces pressed past the bend 

 in some ascended halfway only or after going right up came down 

 again .and stopped halfway. 



Inoculated 13. vi. 14. Incubated 25° C. 



Result 



Dates of Growth on Growth from 



Medium examination surface piece past bend 



Uschinsky's solution 1. vii. 14 Vigorous 



Raulin's fluid 1. vii. 14 Vigorous 



Liquid beef-broth 1. vii. 14 Vigorous 



Liquid beerwort 1. vii. 14 Vigorous 



The results enumerated above clearly show that the fungus is unable 



to take its oxygen from the compounds contained in any of the four 



liquids used. As far as could be determined, the fungus appears to 



be strictly aerobic. 



III. Temperature Relations. 



Tubes of various media were inoculated with the fungus obtained 

 from a beef-broth agar culture, and incubated at the temperature 

 stated in the table below. Two tubes of each medium were incubated 

 at each temperature. 



Date of inoculation: 24. viii. 14. 



Media used and growth at different temperatures 



Note 1. 26. viii. 14. At 30° C. the growth was in every instance more vigorous 

 than at 25° C. 



Note 2. 28. viii. 14. Growth at 25° C. and 30° C. equally vigorous At 15° C. less 

 than at 25° C., and at 37° C. less than at 15° C. 



Note 3. 31. viii. 14. Very little difference between growths at 25° C. and 30° C. 

 on the one hand and 15° C. on the other. At 37° C. no further growth and feeble 

 as compared with growth at 25° C. and 30° C. 



