P. A. VAN DER Bl.JL 



279 



On 31. viii. 14 all the cultures were incubated at 30° C. with the 

 results expressed in the table below. 



Media and growth of cultures exposed abovo at 0° C, 

 37° 0. and 40° C, and from 31. viii. 14 at 30° C. 



IV. 



Beerwort Rico Uschinsky's Sweet 



agar slant tubes solution potato tubes 



Vigorous Vigorous Vigorous Vigorous 

 In all cultures vigorous; about equal to above 

 No growth in any of the cultures 

 No growth in any of tho cultures 



Growth in beef -broth agar of various alkalinities on Fuller's scale 1 . 

 Inoculated 18. v. 14. Incubated 25° C. 



Inoculated 4. vii. 14. Incubated 25° C. 



Note. As the agar did not set firm on the slant, the tubes were not stood upright. 



In reacts — 70 F. to — 100 F. the growth in the presence of sodium 



hydrate was in every case less vigorous than in tubes containing sodium 



carbonate in which latter again the growth was less vigorous than in 



the controls. 



1 Fuller's scale expresses the amount of normal acid or alkali required to neutralise 

 1000 c.c. of an alkali or acid solution. Thus - 15 F. means that the medium is alkaline 

 and that 1000 c.c. requires 15 c.c. of a normal acid to neutralise it. Similarly + 15 F. 

 implies that the medium is acid and that a 1000 c.c. of it will be neutralised by 15 c.c. 

 normal alkali. 



