280 "Wilt" or "Crown-Rot" Disease of Carnations 



V. Growth in Rice of various acidities 1 with Hydrochloric, Nitric and 



Sulphuric Acids. 



In acidities — 30 F., - 60 F. the growth was in three days as vigorous 

 as in the controls. In hydrochloric and sulphuric acid — 80 F., — 105 F. 

 the growth within three days was less vigorous than in the controls, 

 but within five days they came up to the controls. Growth in acidities 

 higher than — 105 F. is given in the tables below. 



Inoculated 14. vii. 14. Incubated 25° C. 



1 Tlio acidity of the rice was determined by placing a known weight of ground rice 

 overnight in a known volume of neutral alcohol. Next morning the alcohol was filtered 

 off, titrated and the acidity determined on Fuller's scale. Five grams of the rice were 

 then placed in a wide test-tube and 5 c.c. of water containing enough acid to produce 

 the required reaction added. The tubes were sterilised on the slant in the autoclave 

 at 110-120° C. for 15 mins. and then inoculated with the fungus mycelium by removing 

 small pieces of agar with the fungus on it and mixing this with the rice. 



- The colour is that noticed ID contact with the glass and the numbers refer to the 

 Repertoire de Couleura published by the Socidte* Franchise <\r* Chrysanthemistes, 



