H. WORMALD 



39 



Five branches were chosen and in all eighteen umbels of flowers were 

 inoculated ; one of these umbels consisted of three flowers, the rest of two 

 flowers each, so that thirty-seven flowers were inoculated. The umbels 

 so treated were mostly at alternate nodes, the intermediate ones being 

 used as controls. Within three days all these flowers showed evidence 

 that infection had occurred, by a brown coloration of the stiomas and in 

 most of them by the discoloration extending also down the style. 



At this stage no other flowers on these branches showed any dis- 

 coloration of the styles or stigmas, thus indicating that the infection was 

 the direct eft'ect of inoculation with the Monilia conidia. The results 

 obtained are given below in detail for Branches I and III, on each of 

 which four umbels of two flowers each were inoculated; these, being also 

 typical of the results obtained on the rest, show the progress of the 

 disease after inoculation of the stigmas until the branch itself is invaded. 



The inoculations were made on May 11 ; the nodes bearing the treated 

 flowers were labelled a, b, c, d. 



Results on the 3rd and ith days after inoculation. 



Branch I. 



May 14 



May 15 



{a) Stigma of each of the two flowers brown Styles brown for 2 and 5 mm. respectively 



(b) Stigma of each flower brown : in one the Styles brown for 2 and 7 mm. respectively 

 discoloration had extended for 2-5 mm. 



down the style 



(c) Stigmas brown; in one the style was Styles broA\Ti for G and 8 mm. respectively 

 also brown for half its length {i.e. for 



about 5 mm.) 

 (f/) Stigmas brown : styles also brown for Styles browTi for 5 and 7 mm. respectively 

 1 and 5 mm. respectively 



Controls: No discoloration, even of the I No discoloration of the stigmas 

 stigmas on the rest of the flowers on the i 

 branch i 



Branch III. 



May 14 



May 15 



(f() Both stigmas brown and both styles : Styles brown: one for 5 inm. the other to the 

 for 2 mm. j " base (about 10 mm.) 



{b) Both stigmas brown; styles brown for i Styles brown for 5 and 7 mm. 

 3 and 4 mm. respectively 



(c) Both stigmas browTi; styles brown for I One style brown for 6 mm. the other to base 

 2-5 and 4 mm. ' 



{(l) Both stigmas brown; style of one 

 flower brown for 2-5 mm. 



Controls: No browTi stigmas 



Style brown for 5 and 8 mm. 

 No brown stigmas 



