212 Studies in Bacteriosls 



II. Cultural Characters. 



The organism grows equally well in mushropni-extract and in beef- 

 extract (Jardox) bouillon +10, and on solid media from these. It also 

 grows luxuriantly upon potato-mush agar. 



Bouillon + 10. Turbidity was strongly marked after 24 hours at 

 22° C. A strong pellicle and faint fluorescence were formed in cultures 

 made during the first two weeks after isolation, but both these characters 

 disappeared in later cultures. The pellicle was easily broken by shaking 

 and did not re-form. A strong pellicle also formed on mushroom-extract. 

 In all cases a slight ring was formed, and after a week or so the liquid 

 was very viscous and mucus-like. It had become strongly alkaline to 

 litmus and smelt of ammonia. 



BouiUon-gelatine Stab. After 48 hours at air temperature liquefaction 

 had commenced, the top 3 mm. being completely liquid with a funnel- 

 shaped depression extending to a depth of some 10 mm. The bottom of the 

 funnel was occupied by a yellowish white granular mass. Growth along 

 the stab was barely perceptible. Complete liquefaction occurred in 

 about ] days, at air temperature. 



BouiUon-gelatine Plate. After 24 hours at 22° C. the colonies had a 

 diameter of 5 or 6 mm. and liquefaction was basin-shaped with a granular 

 deposit at the bottom. In three days the gelatine was completely liquid 

 with a strong ammoniacal odour, resembling somewhat that of stale 

 urine. 



Bouillon-agar Stab. After 24 hours at 22° C. surface growth was 

 yellowish w^hite and about 4 mm. in diameter. Growth along the stab 

 was perceptible only in the top half centimetre. After 72 hours growth 

 could still be seen only towards the top of the stab. The tube was 

 left in the incubator from June 5th till October 2nd, and growth to 

 the bottom of the stab could then be observed, but only by the use of a 

 strong light. The organism was still viable after four months' incubation 

 at 22° C. 



Bouillon-agar Slope. The streak was well developed after 20 hours 

 at 22° C. as a dirty bluish-white wet-shining streak 2 mm. wide, with 

 slightly raised flat contour. When collected in mass by a wire the colour 

 was faintly yellow tinted. The surrounding medium was bluish-green 

 but without fluorescence. 



Bouillon-agar Slope under Anaerobic Conditions. Growth was slight 

 but distinctly perceptible. The organism is a facultative anaerobe with 

 a very marked preference for aerobic conditions. 



