S. G. Paine 213 



Bouillon-agar Plate. Growth was very rapid at air temperature. 

 After 20 hours colonies had a diameter of 1 mm., and after 49 hours of 

 4 or 5 mm. The colonies were round, raised, wet-shininfj. and of a dirty 

 greenish-white colour, the margins later became lobed and spreading. 

 There was no fluorescence but a greenish precipitate was formed in the 

 gel surrounding the colonies. Colonies in the depth of the medium were 

 broadly lenticular. 



Optimum Temperature for Growth. Tubes of bouillon were inoculated 

 each with one loopful from a broth culture and incubated at 6°, 13°, 

 18°, 20°, 22°, 25°, and 30° C. After two days' incubation clouding was 

 most dense at 25° C. ; growth at 6° C. was not apparent until the fifth day. 



Thermal Death Point. Tubes of bouillon were seeded each with 1 cc. 

 of a 24 hours' culture at 25° C. They were immersed in a bath at the 

 usual range of temperatures, the temperature of the tubes being con- 

 trolled by a thermometer immersed in a control tube of broth. Ten 

 minutes heating was allowed at each of the temperatures given below 

 and the tubes were then incubated at 25° C. for seven days. 



III. Physiological Characters. 



The culture used for these experiments Avas the fifth transfer from 

 the original and had been on no other medium than bouillon-agar +10. 

 A tube of bouillon was heavily inocvdated and a loopful from this used 

 for each test. The temperature of incubation was 22° C. 



10 per cent. Witte Peptone + 1 per cent. Glucose. Acid on the second 

 day, no gas formed in Durham tube; no change after 15 days. 



10 per cent. Witte Peptone + 1 per cent. Lactose. No acid and no gas; 

 a thin film with an oily appearance, a slight ring formed and liquid 

 became slightly turbid. 



10 per cent. Witte Peptone + 1 per cent. Saccharose. No acid and no 

 gas; growth as above. 



Bouillon + 2 per cent. Glucose. Acid after 24 hours, no gas, no 

 pellicle, no ring; no further change up to 15 days. 



