214 Studies in Bacteriosis 



Bouillon + 2 ^^er cent. Lactose. No acid, no gas, slight pellicle, no 

 ring. 



Bomllon + 2 per cent. Saccharose. No acid, no gas, slight pellicle, no 

 ring. 



Bouillon + 2 per cent. Mannite. No acid, no gas, no pellicle, no ring; 

 slightly bleached at bottom of tube after 15 days. 



UscJiinshfs Solution. Alkaline on second day, no gas, loose pellicle, 

 no fluorescence. After 15 days: completely bleached, no gas, no fluor- 

 escence, thin pellicle and strong ring. After two months: blue above, 

 bleached below, no fluorescence, very viscous deposit. After four months : 

 the ultramarine colour had completely returned. 



Litmus Milk. Curd separated after the fourth day, the reaction was 

 neutral but became slightly alkaline later. On the seventh day the 

 curd occupied only one-third of the volume of Hquid, the litmus was 

 bleached and a strong green fluorescence developed in the whey. Most 

 of the curd was digested but some remained at the bottom of the tube 

 after six weeks. 



Milk. The curd formed and was partially digested as above, and the 

 whey became strongly fluorescent. 



Potato Plug. After two days the streak was visible as a white, wet- 

 shining, raised mass; after five days the colour had assumed a faint 

 yellow tinge ; the potato was not discoloured. Pulped in water after 

 three weeks, the iodine test showed slight dia^tatic action. 



Dunham's Solution. Became slightly turbid on the second day; no 

 pellicle and no ring formed. After 30 days a trace of indol was present. 



Nitrate Bouillon. A loose pellicle formed after 24 hours, the liquid 

 became very viscous and of a yellowish-green colour. Nitrate was not 

 produced, but ammonia was formed. This was probably a product of 

 the breakdown of protein since nitrate was still present after two months' 

 incubation. The presence of nitrate was not due to the death of the 

 organism previous to destruction of all the nitrate since the culture was 

 found to be still viable at the end of two months. No gas was formed. 



GENERAL DISCUSSION, COMPARISON OF THE ORGANISM 

 WITH ALLIED STRAINS. 



In searching for the mode of entry of the organism to the sheds the 

 possible sources to be considered were: (1) the manure used in making 

 the beds; (2) the spawn; (3) the mould used for casing; (1) the straw 

 litter for covering the beds after spawning: (5) thp water used for 

 sprinkling; and (G) the air. Suspicion of the manure was removed by 



