Sydney G. Paine amd Margaret S. Lacey 



211 



Glucose broth 



Lactose broth 



Sucrose broth 



Potato plug 



Diastatic action ... 

 Milk tubes 



Indol formation 

 Staining 



Size and shape 

 Motility 



Aplanohacler (Paine 

 and Bewley) 



Slight acid on 6th day lit- 

 mus reduced, pellicle 



Slight acid on 9th day lit- 

 mus reduced, pellicle 



Slight acid on 6th day lit- 

 mus reduced, pellicle 



Thick viscous growth, wet 

 shining, deep orange, po- 

 tato dark grey 



Strong 



No clotting, digestion of 

 casein apparent after 9 

 days, comjilete in 3 

 weeks 



Slight sign 



Gram positive when first 

 isolated but later became 

 gram negative 



Small oval rods, 1-6^ x 0-6fi 



Non-motile 



Aplanobader 

 michiganense E.F.S. 



Slight acid on 13th day 

 litmus not reduced, no 

 pellicle 



Slight acid on 13th day 

 litmus not reduced, no 

 pellicle 



Slight acid on 13th day 

 litmus not reduced, no 

 pellicle 



Growth not nearly so vis- 

 cous, pale yellow, potato 

 pinkish 



Strong 



No clotting, casein diges- 

 tion not apparent until 

 the 14th day, only slight 

 after 30 days 



No sign 



Gram positive 



Small oval rods, l-6jU x 0-6ja 

 Non-motile 



From the above it is seen that while these two organisms possess 

 many properties in common, certain differences, mainly differences 

 in degree only, clearly mark them as different species and the name 

 Aplanohacter dissimulans is now proposed for the species isolated by 

 Paine and Bewley. 



Infection Experiments. 



Inoculations of young tomato plants were carried out in the experi- 

 mental houses at Waltham Cross by Dr W. F. Bewley, to whom the 

 authors tender their best thanks. The two Aplanobacters and Bacillus 

 latkijri were pricked separately into three sets of eighteen plants; the 

 results with Ajjlanobacter dissimulans were negative in every case; many 

 successful infections were obtained with B. laihyri and A. michiganense, 

 the effect upon the pith was identical and altogether indistinguishable, 

 but marked differences were observed when the organisms, having passed 

 through the cortex, produced lesions on the exterior of the stem. The 

 former produced dark brown sunken furrows with usually no cracking 

 of the epidermis, while the latter gave no special colouring but produced 

 deep fissures in the outer cortex whose margins had the appearance of 

 callus formations ; no effect upon the fruits was observed in the case of 

 Aplanohacter michiganense. The two diseases therefore appear to be 

 entirely distinct, and the senior author desires to withdraw the suggestion 



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