OOKINESIS IN LIMAX MAXIMUS. 381 



EXPLANATION OF PLATE XVII. 



Fig. 1. — Section of a sperm ogonium, x 200. 



2. — Section of an tecidium, x 150. De Bary. 



3. — /Ecidiospore germinating, x 250. 



4. — Teleutospore germinating. 



5. — Promycelial spore germinating. 



6. — Paris quadrifolia, showing secidium on under surfaces of leaves. 



7. — Glass slip, with india rubber cell. 



©bftineeie in Ximay flDayimue/' 



By F. L. Washburn. 



THE observations here given are confined to early stages of 

 the egg while in the oviduct, and before the expulsion of 

 either polar globule. The article, therefore, deals with 

 stages which, for the most part, precede any discussed by Dr. Mark 

 in his excellent treatise on Z. campestris.\ 



Of the following wood-cuts, Fig. i is a diagrammatic represen. 

 tation of the oviduct from a laying animal, from which eggs were 

 taken, and studied serially as numbered. The vitellus averaged 



ft,^ 



Fig I. 

 i56"2^ in diameter. Various methods were made use of in fixing 

 — Fols solution : Osmic acid, i per cent., followed by Merkel's 

 fluid j chromic acid 1/3 per cent., etc. ; but the one which gave 



* Reprinted from American Nahcralist, June, 1894. 



t "The Maturation, Fecundation, and Segmentation of Limax Catnpestris 

 Binney," by E. L. Mark, Bulletin of the MtiseuDi of Compaj-ative Anatomy, 

 Vol. VL, parts 11 and 12, Cambridge, Mass., 1881. 



