OOKINESIS IN LIMAX MAXIMUS. 



383 



which Garnault "^ says do exist in the ovarian egg of Arion and 

 Helix^ and which were not seen in the hermaphrodite gland of L. 

 ??iaxifmis, do exist in the duct very near the gland. They evidently 

 appear immediately after the egg has left the ovary. This duct 

 was lined, for the most part, with ciliated epithelium, and con- 

 tained much mucus. 



Fig. 2 illustrates an optical section of egg No. i from glandular 

 part of the oviduct (see Fig. i) viewed obliquely to the long axis 

 of the spindle, and showing the two polar corpuscles and chromo- 

 somes, there being about twenty of the latter lying in an irregular 

 cluster in the clear space between the corpuscles. This egg was 



Fig. 2. 



stained in picrocarminate of lithium for thirty hours. In its exam- 

 ination a Zeiss Oc. 2 and Obj. E were used. A broken membrane, 

 " membrane rongee," was seen with apparently chromatic thicken- 

 ings in it. Observations on this egg coincide closely with those of 

 Garnault on Arion and Helix ^ and, in a measure, with those of 

 Vejdovsky on Rhynchebnis.\ 



The larger corpuscle is the one nearest the observer. The 

 structural peculiarity of one side of the nucleus should be noted — 

 where cytoplasm and yolk granules are in intimate relation with 

 contents of nucleus. This is Garnault's " prophase ; " it is the 

 stage just previous to formation of nuclear plate leading to the 

 forming of first polar globule. In another egg, No. 9, from the 

 same oviduct, an optical section showed rays of hyalocytoplasm 



* " Sur les phenomenes de la fecundation chez I'Helix aspersa et I'Arion 

 empiricorum." — Zool. Anzeiger^ Nos. 297 & 298, Dec, '88, and Jan., '89. 



t Die Entivecklungsgeschicete der oligochaeten ( Rhynchelniis), 1888. 



