TABLE OF CONTENTS* 



(May 1949) 

 Leaflet I. Introductory 



Purpose of the Manual 

 Historical 



I44-2 Use of the Manual 

 I44-5 Glossary 



-9 



-13 



Leaflet IL Preparation of Media 



Sterilization "44-3 



Cultivation and storage media 1I44-4 



General differential media 1I44-6 



Media for special groups of 



aerobes 1I44-9 



Media for anaerobic bacteria ii.j-14 



Leaflet III The Study of Obligately Anaerobic Bacteria 



(Introduction) 



Biological methods for oxygen 



removal 

 Chemical methods for oxygen 



removal 

 Oxygen removal by combustion 



using Laidlaw principle 



IIl4,-3 



iii,,-10 



Plating system using strongly 

 reducing medium 



Preliminary microscopic exami- 

 nation 



Microscopic examination of 

 pure cultures 



Cultivation technics 



11I43-I2 



1114,-14 



11I43-I4 



IIl4,-16 



Other methods of value 



iii4,-20 



Leaflet IV. Staining Methods 



General principles IV46-3 

 General bacterial stains 



Recommended procedures IV46-5 



Alternate procedures IV46-6 



Negative staining of bacteria IV46-7 



The Gram stain IV46-8 



Acid-fast staining IV46-IO 

 Spore staining 



Recommended procedures IV46-II 



Alternate procedures IV46-I3 



Staining the diphtheria organism 

 Recommended procedures 

 Alternate procedure 



Flagella staining 



Capsule stains 



Stains for Spirochaetes 



Stain for Rickettsiae 



Dye solubilities 



IV46-I3 

 IV46-I4 

 IV46-I5 

 IV46-I8 

 IV46-2O 

 IV46-2O 

 IV46-2I 



Leaflet V. Routine Tests for the Descriptive Chart 



*In these page numbers, the Roman numerals refer to the leaflet, the small inferior 

 numerals to the year of the edition, and the large arable numerals to the page of the leaflet. 



