STANDARD DESCRIPTIVE CHART 



,; ine of organism Source Studied by 



,10 of isolation Habitat Optimum conditions: Media 



phase variation observed? Phase on this Chart: S, R, M, G (smooth, rough, mucoid, gonidial) Phases recorded on other charts: 



..Culture No... 

 Temp... 



Jerscore required terms. 



..Age 



xTATivE Cells: Medium used 



Reaction (pH) Temp 



: eof Majority 



tiids. rounded, truncate, concave, tapering 



yiLiTY: In broth On agar 



f;ANGiA and Endospores: present, absent. 



Medium used pH Temp Age c 



Endospore Form: spherical, ellipsoid, cylindrical 



ji-rLAR Forms: 

 P'rsent on in days at "C 



:,:R Colonies; Temperature "C. Age d 



r -rm, punctiform (i. e. under i m.m. diam.), circular (i. e. ove\ 



J m.m. diam.) , filamentous, irregular, rhizoid. 

 ^' irface, smooth, rough, concentrically ringed, radiately ridged 

 ^jj^c. entire, undulate, lobate, erose, filamentous, curled. 

 r;. vation of growth, effuse, fiat, raised, convex. 

 Optical Characters, opaque, translucent, opalescent, iridescent 



Gelatin Colonies: Temperature °C. Age d 



Form, punctiform, circular, irregular, filamentous. 



E evation, fiat, raised, convex, pulvinate, crateriform (Hque- 



fyttig). 

 E'ige, entire, undulate, lobate, erose, filamentous, curled. 

 ijnuefaction, cttP, saucer, spreading. 

 rtace, smooth, contoured, rugose. 

 :iticai Characters, opaque, translucent, opalescent, iridescent. 



; Stroke: 



Temperature °C. Age d, 



I . wth, scanty, moderate, abundant, none. 



n of growth, filiform, echinula te, beaded, spreading, 

 .:, horescent, rhizoid. ^— ii^ 



. ire, glistening, dull. 



iromogenesis photogenic, fluorescent. 



I Mr, absent, decided, resembling.. 



( nsistency, butyrous, viscid, membranous, brittle. 

 Medium, grayed, browned, reddened, blued, greened, unchanged. 



NiTRiENT Broth: Temperature "C. Age c 



Surface growth, ring, pellicle, fhcculent, membranous, none. 

 Clouding, slight, moderate, strong, transient, persistent, nofie, 

 Jlutd turbid, granular growth. 



<Jd ir, abse7it, decided, resembling 



S-Jiment, compact, fiocculent, granular, flaky, viscid. 

 .'vri.ount of sediment, abundant, scanty, none. 



jELATiN Ptab: Temperature °C. Age d. 



Growth, uniform, best at top, best at bottom. 



Line of puncture, ^ii/orjn, beaded, papillate .villous , arbor escent . 



Liquefaction, none, crateriform, infundibuHform, napiform, 



saccate, stratiform: begins in d. complete in d. 



Degree of liquefaction in days 



Method used 



^Medium, fluorescent, browned, unchanged. 



Surface Colonies 



Surface Colonies 



yy 



Medium: 

 Teraperatui 



Age 



Medium: 

 Temperatu: 



Medium: 

 Temperatun 



FERMENTATION 



Temperature °C. 



Medium 



staining.. 



.and: 



J^^^mcntation liibe 



'•""• CO, in Eldredge tube 



^Mion {pH) after d. 



^'•'"bU acidity in 

 Jii::: NaOH 



Monosaccharides 



Disaccharides 



Polysaccharides 



Alcohols 



Glucosides 



BRIEF CHARACTERIZATION 



As each of the following characteristics is determined, indicate in proper marginal 

 square by means of figure, as desi^ated below. In case any of these characteristics are 

 doubtful or have not been determined, indicate with the letters U, V, and X according 

 to the following code: 



U. undetermined; V, variable; X. doubtful. 



Form & arrangement: 1, streptococci; 2, diplococci; 3, micrococci; 

 4. sarcinae; 5, rods; 6, commas; 7, spirals; 8, branched rods; 9, filamentous 



Diameter: 1, under 0.5/i; 2, between 0.5)u and 1/*; 3, 



Gram stain: 0, negative; 1, positive 



i:0, absent; 1, peritrichic; 2, polar; 3, present but undetermined 



Capsules: 0, absent; 1, present 



Chains (4 or more cells) : 0. absent; 1, present 



Sporangia: 0, absent; 1 , elliptical ; 2, short rods : 3, spindled ; 4, clavate; 5, drumsticks 



Endospores: 0, absent; 1, central to excentric; 2, subterminal; 3, terminal 



Growth: 0, absent; 1, abundant; 2, moderate; 3, scanty 



Lustre: 1, glistening; 2, dull 



Form: 1, punctiform; 2. circular (over 1 mm. diameter); 

 3, rhizoid; 4, filamentous; 5, curled; 6, irregular 



Surface: 1, smooth; 2, contoured; 3. rugose 



Form: 1, punctiform; 2, circular (o 



1.); 3, irregular; 4, filamentous 



Surface: 1, smooth; 2, contoured; 3, rugose 



Biologic relationships: 1, pathogenic for man; 2, for animals but not for n 

 3, for plants; 4, parasitic but not pathogenic; 5, saprophytic; 6, autotrophi( 



Relation to free oxygen: 1, strict aerobe; 2, facultative anaerobe; 3, strict 

 anaerobe; 4, microaerophile 



In nitrate media : 0, neither nitrite nor gas; 1 , both nitrite and gas; 2, nitrite but 

 no gas; 3, gas but no nitrite 



Chromogenesis: 0, none; 1, pink; 2, violet; 3, blue; 4, green; 5, yellow; 

 6, orange; 7. red; 8. brown; 9, black 



Other photic characters: 0, 



; 1, photogenic; 2, fluorescent; 3, iridescent 



Indole: 0, negative; 1, positive 



Hydrogen sulfide: 0, negative; 1, positive 



Hemolysis: 0, negative; 1, positive 



Methemoglobin: 0, negative; 1, positive 



Protein 

 Liquefac- 

 tion or 



Digestion 



Gelatin: 0, negative; 1, positii 



Casein :0, negative; 1, positive 



Egg albumin : 0, negative ; 1 , positive 



Blood serum: 0, negative; 1, positive 



Indicator 

 Reduc- 

 tion 



Litmus : 0. negative ; 1 , positive 



Methylene blue: 0, negative; 1. positive 



Janus green: 0, negative; 1, positive 



Rennet production: 0, negative; 1, positive 



