SUPPLEMENTARY DATA 



TEMPERATURE RELATIONS 



Medium PH 



Optimum temperature for growth ^• 



Maximum temperature for growth °C. 



Minimum temperature for growth °C. 



Thermal death point: Time 10 minutes: °C. 



Medium pH 



Thermal death time: 



Medium "• PH 



Temp. 



Time 



...min. 



Temp. 



"C. 



"C. 



Time 

 ...min 

 ...min 



CHROMOGENESIS 



Gelatin 



Agar 



Potato 



OTHER PHOTIC CHARACTERS 



Photogenesis on 



Iridescence on 



Fluorescence in 



RELATION TO REACTION (pH) OF MEDIUM 



Medium 



Optimum for growth : oioM/ />if 



Limits for growth :/i-om pH to 



RELATION TO FREE OXYGEN 



Method 



Medium Temp °C. 



Aerobic growth: absent, present, better than anaerobic growth, 



micro-aerophilic 

 Anaerobic growth: absent, occurs in presence of glucose, of 



sucrose, of lactose, of nitrate; better than aerobic growth 

 Additional data: 



Reaction: 

 Acid curd: 

 Rennet curd: 

 Peptonization: 



Reaction: d. . 



Acid curd: d. . 



Rennet curd: d. . 



Peptonization: d. . 



Reduction of litmus beg] 



LITMUS MILK 



Temperature *^C. 



d ; d ; d 



..days, ends in days 



ACTION ON ERYTHROCYTES 



Cells: 



Method: plate, broth, filtrate 

 Hemolysis: negative, positive 

 Methemoglobin: negative, positive 



PRODUCTION OF INDOLE 



Medium 



Test used 



Indole absent, present in days 



PRODUCTION OF HYDROGEN SULFIDE 



Medium 



Test used 



H.S absent, present in days 



ACTION ON NITRATES 



Medium Temp °C. 



Nitrite: d ; d ; d ; d 



Gas(N.): d ; d ; d ; d 



Medium Temp °C. 



Nitrite: d : d ; d ; d 



Gas(N.): d ; d ; d : d 



Ammonia production (in amino-N-free nitrate medium): 



negative, positive 

 Complete disappearance of nitrate in medium: 



negative, positive 

 Disappearance of 2 p. p.m. nitrite in medium: 



negative, positive 



REDUCTION OF INDICATORS 



Medium pH Temp ^ 



Indicator Cone. Reduction: 



% hr ; hr..... 



% hr ; hi. " 



% hr ; hi." 



% hr ; hr. ' 



Gram: d. ... 



Method 



Spores: Method 



Capsules: Method 



Medium 



Flagella: Method.. 

 Special Stains: 



STAINING REACTIONS 



; d ; d. ... 



ADDITIONAL TESTS 



Methyl red: negative, positive 



Voges-Proskauer: negative, positive 



Growth in sodium citrate: absent, present 



Growth in uric acid : absent, present 



Hydrolysis of starch: complete (iodine colorless): iirU 



(iodine reddish-brown): none (iodine blue) 

 Nitrogen obtained from the following compounds: __ 



PATHOLOGY 



*In each instance where pathogenicity is observed, indicate location of lesion, and type, e. g. edema, histolysis, gas, 

 hemorrhage, ulcer, diphtheritic, etc. 



ANTIGENIC ACTION 



Ammal Medium used Age of culture 



Type injection Number of injections 



Culture causes production of cytolysins, aggulutinins, precipitins, antitoxin. 



Specificity: Antibodies produced effective against other antigens as follows 



Immune sera from.. 



..efEective against this organism as antigen 



SPECIAL TESTS 



This Descriptive Chart presented at the annual meeting of the Society of American Bacteriologists, Dec. 28, 1934. by the Committee on Bacteriological Technic. 

 Prepared by a sub-committee consisting of M. W. Jennison and H. J. Conn. 



