VII48-14 MANUAL OF METHODS FOR PURE CULTURE STUDY 



of the methods that few bacteriologists possess the technical knowl- 

 edge or skill to apply them satisfactorily. 



Summary 



It is obvious that suitable technic, skilfully applied, and extreme 

 caution in interpretation of the results are necessary to determine the 

 pathogenic properties of microorganisms. Indirect evidence is ac- 

 ceptable as a substitute when Koch's postulates are inapplicable or 

 when animal inoculation experiments are not entirely satisfactory; 

 but such evidence is rarely sufficiently conclusive except as a working 

 hypothesis. In the absence of conclusive evidence, the organism 

 should be considered pathogenic only so far as the experiments 

 indicate. 



To facilitate study of an investigation by others the methods used 

 should be reported punctiliously. 



REFERENCES 



BuRKY, Earl L. 1934. Production of lens sensitivity in rabbits by the action of 

 staphylococcus toxin. Proc. Soc. Exp. Biol. & Med., 31, 445. 



Chapman, George H. 1945. Staphylococci in gastroenterology. Am. J. Digestive 

 Dis., 12, 399. 



Ch.-vpman, George H. 1946. The staphylococci. Trans. N. Y. Acad. Sci., 9, 52. 



Chapman, George H. 1947. Relationships of nonhemolytic and viridans strepto- 

 cocci in man. Trans. N. Y. Acad. Sci., 10, 45. 



Gumming, C. N. Wentworth. 1943. The importance of Mus musculus in research: 

 A discussion of its genetic aspects. Presented before the Am. Public Health 

 Assn., New York, Oct. 11-14. 



Cumming, C. N. Wentworth. 1947. Modern mass production in animal breeding 

 for experimental research. In press. Read before the Fourth International 

 Cancer Research Congress. 



De Angelis, Eugene. 1937. A reaction with iron compounds for the determination 

 of B. anthracis and of its pathogenicity. J. Bact., 33, 197. 



Dozois, K. Pierre and Rauss, K. F. 1935. Relationship between electrophoretic 

 migration velocities, the virulence and the types of the diphtheria and 

 diphtheria-like bacilli. Am. J. Pub. Health, 25, 1099. 



DuBOS, Rene J. 1945. The Bacterial Cell In Its Relation to Problems of Virulence, 

 Immunity and Chemotherapy. Harvard Univ. Press. 



Elliott, S. D. 1938. The use of saponin in blood culture media, with special refer- 

 ence to blood cultures in subacute bacterial endocarditis. J. Path. & Bact., 

 46, 121. 



Farris, Edmond J., Carnochan, F. G., Cumming, C. N. W., Farber, Sidney, Hart- 

 man, Carl G., Hutt, Frederick B., Loosli, J. K., Mills, Clarence A. and 

 Ratcliffe, Herbert L. 1945. Animal colony maintenance. Ann. New 

 York Acad. Sci., 46, (Art. 1) 1. 



Feder, F. M. 1937. A new and simplified blood culture technic. J. Lab. & Clin. 

 Med., 22, 846. 



Felton, Lloyd D. 1932. Studies on Virulence: Influence on virulence of pneumo- 

 cocci of growth on various media. J. Exp. Med., 56, 13. 



Fulton, F. 1943. Staphylococcal enterotoxin — with special reference to the kitten 

 test. Brit. J. Exp. Path., 24, 65. 



Hanger, Jr., Franklin M. 1928. Effect of intravenous bacterial filtrates on skin 

 tests and local infections. Proc. Soc. Exp. Biol. & Med., 25, 775. 



Holman, W. L. and Carson, Arline E. 1935. Technical errors in studies of bac- 

 terial variations. J. Inf. Dis., 56, 165. 



Kracke, Roy R. and Teasley, Harry E. 1930. The efficiency of blood cultures. 

 J. Lab. & Clin. Med., 16, 169. 



McLeod, J. W. and Bevan-Brown, R. E. 1918. The technique of blood culture. 

 J. Path. & Bact., 22, 74. 



