INOCULATIONS WITH BACTERIA CAUSING PLANT DISEASE x^-lS 



Table 1. Tentative Protocol for Plant Inoculations 



Host: Manner of inoculation: 



Variety Through soil 



History Through wounds 



Age Ry sprays 



]Mon)hological condition Spreader used .... 



Physiological condition By insects (name) . . 



Susceptibility ^ , Stage in life cycle . 



Enyironment Other means 



Treatment before . 

 Treatment after.. 



Incubation: 



Time 



Enyironment: 

 Pathogen : Temperature . 



Strain Moisture . 



History Light . 



Culture on Soil nutrients . 



at °C. 



for days Symptom.s : 



Location 



J , J Age of parts affected , 



Inoculum used: c t 



Severity . 



Diseased tissue Description : ' 



Entire culture 



Early 



Bacteria: Medmm , 



Turbidity Final . . . 



Number per cc 



Filtrate Effect on yield: 



Products Quantity .... 



Amount used per plant Quality 



INTERPRETATION OF RESULTS 



The results of research are vaHd only in accord with the reliability 

 of the methods employed and the accuracy of their interpretation. 

 After an experiment has been performed it is insisted that a report 

 of such work must not be published for the use of others until repeated 

 determinations have been made and the results have been satisfac- 

 torily analyzed. The simpler experiments are commonly performed 

 with suitable controls at least in duplicate or triplicate, and carried 

 through three separate times. A good investigator does not become so 

 enthusiastic about an experiment that he fails to view it impartially 

 and to accept sound evidence against it. On the contrary, he makes 

 every reasonable effort before publishing to find an error in the experi- 

 ment itself or in the conclusions drawn from it. 



