T AGGLUTININ 



merits, the agglutinin disappeared immediately but was 

 present in four to eight times normal titre on the sixth and 

 eighth day, returning to normal about the twenty-first. 

 Associated with the rise there was an appearance of cold 

 agglutinin for human cells (French and Ada, 1954). 

 Fraser (1955) using rabbit cells treated in vitro found no 

 response when these were injected into normal rabbits, 

 presumably because the dose was much lower than in 

 French and Ada's guinea-pigs. He could, however, confirm 

 Burnet and Anderson: ( 1947), in that treated heterologous cells 

 gave a brisk and specific response. The latter showed that 

 with fully treated guinea-pig or human cells, there was 

 a sharp diminution in the production of agglutinin for normal 

 cells and the appearance of a specific 'immune T' agglu- 

 tinin. Table 3 from Burnet and Anderson shows a typical 

 protocol to establish this. 



Table 3. Behaviour of 'immune T' agglutinin 

 {Burnet and Anderson, ig4y) 



NH = Normal human red cells. 

 RDH = Human cells treated with RDE. 



Fraser (1955) was interested in the changes associated 

 with warm and cold agglutinin titres. The normal T agglu- 

 tinin of the rabbit contains a considerable proportion of less 

 avid 'cold' agglutinin which cannot be absorbed at 40° by 

 RD cells. On immunization with heterologous treated cells, 

 a high T agglutinin was not associated with any cold 

 agglutinin which, however, reappeared 6 to 1 2 months later. 



7 97 Bc 



