no Journal of Agricultural Research voi. xxii, no. 2 



The ratio of nitrogen to sulphur in the portion of the clover plant 

 insoluble in dilute acetic acid remains about the same, regardless of the 

 stage in the development of the plant. This gives further support to the 

 view that the nitrogen insoluble in acetic acid represents protein nitrogen. 

 The total nitrogen and total nitrogen insoluble in acetic acid wias higher 

 in those plants cut before the blossoming stage. 



With clover growing on sand cultures, it was possible, by reducing the 

 available nitrate, not only to limit the growth and nitrogen content but 

 also to decrease the sulphur assimilation. So, while sulphates appar- 

 ently cause greater nitrogen assimilation through their beneficial effect 

 on nodule development, the amount of sulphur taken up by the plant is 

 limited by the total nitrogen absorbed. 



The rape plant assimilated a large amount of sulphur, although the 

 presence of sulphates reduced the yield compared to the control soil 

 cultures. Sulphate plus nitrate caused increased yields compared with 

 those secured when nitrate was added alone. There does not appear 

 to be any direct relation between nitrogen and sulphur assimilation in 

 the rape plant. 



LITERATURE CITED 

 (i) Arny, a. C, and Thatcher, R. W. 



1915-17. THE EFFECT OF DH'FERENT METHODS OP INOCULATION ON THE YIELD 

 AND PROTEIN CONTENT OP ALFALFA AND SWEET CLOVER. In JOUT. Amer. 



Soc. Agron., v. 7, no. 4, p. 172-185, 1915; v. 9, no. 3, p. 127-137. 

 1917. 



(2) DULEY, F. L. 



1916. THE RELATION OF SULFUR TO SOIL PRODUCTIVITY, /n JoUT. Amer. Soc. 



Agron., V. 8, no. 3, p. 154-160. 



(3) Hart, E. B., and Peterson, W. H. 



191 1. SULFUR REQUIREMENTS OF FARM CROPS IN RELATION TO THE SOIL 



AND AIR SUPPLY. Wis. Agr. Exp. Sta. Research Bui. 14, 21 p. 



(4) Miller, H. G. 



I9I9. RELATION OF SULFATES TO PLANT GROWTH AND COMPOSITION. In Jour. 



Agr. Research, v. 17, no. 3, p. 87-102, pi. 9-12. Literature cited, 

 p. 100-102. 



(5) PiTz, Walter. 



1916. EFFECT OF ELEMENTAL SULFUR AND OF CALCIUM SULFATE ON CERTAIN 



OF THE HIGHER AND LOWER FORMS OF PLANT LIFE. In JoiW. AgT. 



Research, v. 5, no. 16, p. 771-780, pi. 56. 



(6) Prucha, Martin J. 



1915. PHYSIOLOGICAL STUDIES OP BACILLUS RADICICOLA OF CANADA FIELD PEA. 



N. Y. Cornell Agr. Exp. Sta. Mem. 5, 83 p. Bibliography, p. 79-83. 



(7) Reimer, F. C, and Tarter, H. V. 



I919. SULFUR AS A FERTILIZER FOR ALFALFA IN SOUTHERN OREGON. Oreg. 



Agr. Exp. Sta. Bui. 163, 40 p., 9 fig. Bibliography, p. 39-40. 



(8) Wn^ON, J. K. 



1917. PHYSIOLOGICAL STUDIES OF BACILLUS RADICICOLA OF SOYBEAN (SOJA 



MAX piper) AND OF FACTORS INFLUENCING NODULE PRODUCTION. 



N. Y. Cornell Agr. Exp. Sta. Bui. 386, p. 363-413, fig. 80-94. Bibli- 

 ography, p. 412-413- 



