1^2 Journal of Agricultural Research v^oi. xxii. No. 3 



aecia on Anchusa, and when sowings were made with aeciospores from 

 Anchusa, uredinia on rye were developed. Sowings of basidiospores from 

 rye upon Berheris vulgaris Hoi., Rhamnus Frangida, Rhamus cathartica, 

 Ranunculus acris, Ranunculus bulbosus, Taraxacum officinalis, and Urtica 

 dioica were without result. Nielsen {20, p. 37) 10 years later reported 

 obtaining infection with aeciospores from Anchusa officinalis on both rye 

 and wheat. Plowright {21, p. 168) states that in the fall of 1885 he 

 obtained aecia upon Anchusa arvensis by placing wheat straw rusted 

 with P. rubigo-vcra near that host. 



At the time this work was carried out the name Puccinia rubigo-vera 

 was used for the leafrusts of wheat, rye, and barley, as well as for similar 

 grass rusts having globoid urediniospores and long covered telia. 

 Eriksson and Henning (11, p. 197-203, 2^7-2^9) separated this species 

 into two — Puccinia glumarum, the stripe rust, and Puccinia dispersa 

 Eriks., the brown rust. Under the latter they included the rust of wheat 

 as well as that of rye. The rust of wheat, however, was considered as a 

 forma specialis, Tritici, of Puccinia dispersa. As the leafrust of rye had 

 been shown by De Bary (4) to be connected with aecia on Anchusa, 

 Eriksson (jo, p. 254-2^7) sought for the same connection for the leafrust 

 of wheat. His sowings of basidiospores from wheat upon Anchusa 

 officinalis and A. arvensis, however, produced no infection, as was also 

 the case when aeciospores from Anchusa were sown on wheat. No results 

 were obtained when basidiospores were sown on Nonnea rosea, Myosotis 

 arvensis, M. alpestris, Symphytum asperrimum and Pulmonaria officinalis, 

 species of Boraginaceae related to Anchusa upon which unconnected 

 aecia were known to occur. As a result of these cultures, Eriksson {10, 

 p. 270) concluded that the orange leafrust of wheat was a distinct species 

 and gave it the name, Puccinia trificina. 



Klebahn (17, p. 85-86; 18, p. 246) made rather extensive cultures in 

 an endeavor to discover the aecial host of Puccinia triticina. Besides 

 sowing aeciospores of Aecidium Anchusae Eriks. and Henn. on wheat he 

 made sowings of basidiospores on Anchusa arvensis and Anchusa officinalis 

 without result. Sowings of basidiospores also were made without success 

 upon Triticum vulgare, Ranunculus acer, Ranunaihis asiaticiis, Ranun- 

 culus auricomus. Ranunculus bulbosus, Ranunculus Ficaria, Ranunculus 

 flammula, Rammculus lanuginosus , Ranunculus repens, Anemone ranun- 

 culoidcs, Aconitum Lycoctonum, Aconitum Napellus, Berberis vulgaris, 

 Nasturtium sp., Barbaraea vulgaris, Melandryum albutn, Coronaria fl.os- 

 cuculi, Agrostetnma Githago, Rhamnus cathartica, Lythrum Salicaria, 

 Ribes Grossularia, Aegopodium Podagraria, Pastinaca sativa, Valeriana 

 dioica, Knautia arvensis, Tussilago Farfara, Taraxacum, officinale, Cen- 

 taurea Cyanus, Achillea Ptarmica, Campamila rotundifolia, Ligustrum 

 vulgare, Phillyrea sp., Echium vulgare, Lithospermum purpureo-coeruleum, 

 Myosotis sp., Symphytum officinale, Glechoma hederacea, Prunella vulgaris, 



