Nov. 19, 1921 



Hemotoxins from Parasitic Worms 



409 



Table III gives a record of the experiments performed with this extract. 



Table III. — Effect of extract II of fresh worms (Ancylostoma caninum) on washed red 



blood corpuscles « 



Kind of corpuscles. & 



Rabbit 

 Do 

 Do 



Dog... 

 Do 

 Do 



Hog..., 

 Do 

 Do 



Cattle. . 

 Do 

 Do 



Quantity of extract. 



5 drops. . 

 8 drops. . 

 Control c . 

 5 drops. . 

 8 drops. . 

 Control c. 

 5 drops. . . 

 8 drops. . , 

 Control c. 

 5 drops. ., 

 8 drops. . , 

 Control*:. 



Period of incubation. 



1 hour. . . . 

 ....do... 

 ....do... 



2 X hours. 

 ....do... 

 ....do... 

 ....do... 

 ....do... 

 ....do... 

 ....do... 

 ....do... 

 ....do... 



Results at 

 end of in- 

 cubation 

 period. 



+ + + 

 + + + 



+ + 



Results 

 after 18 

 hours addi- 

 tional in re- 

 frigerator 

 (S^C). 



+ + 

 + + + 



+ + + 

 + + + 



o ++ indicates marked though incomplete hetaolysis. -H-+ indicates complete hemolysis. — indi- 

 cates absence of hemolysis. 



''0.2 cc. of a 5 per cent suspension of washed blood corpuscles were used in all experiments summarized 

 in this table. 



" Eight drops of physiological salt solution were added to the washed blood corpuscles in order to control 

 the experiment. 



The sediment in the tube containing the extract of hookworms when 

 shaken with 3 cc. of physiological salt solution yielded additional hemoly- 

 sis, as the following experiments will show. 



Experiment 7.— After the supernatant fluid from the extract (extract 

 II of fresh worms) had been removed the sediment was shaken up with 

 about 3 cc. of physiological salt solution, v/hich was tested against a 5 

 per cent suspension of washed dog corpuscles from the same lot as that 

 referred to in Table I. Three drops of corpuscles were completely 

 hemolyzed by three drops of the extract after one hour's incubation at 

 37° C. This experiment was controlled as usual. 



Experiment 8. — Five drops of the same extract were boiled for about 

 one minute. After cooling, three drops of dog erythrocytes from the 

 same lot as that used in experiment 7 were added and the mixture 

 incubated for 1% hours at 37° C. No hemolysis was produced. The 

 tube was kept 18 hours in the refrigerator without any change. 



EXPERIMENTS WITH EXTRACTS OE DRIED WORMS 



The experiments recorded below were performed with the following 

 extract : 



Fifty-eight mgm. of coarsely powdered worm material {Ancylostoma 

 caninum) dried at 37° C. and kept in a small vial for about two years were 

 extracted in 10 cc. of physiological salt solution for several hours. Unlike 



