494 Agricultural Journal of Victoria. 



Gelatine Cultures. — On gelatine plates the surface colonies are 

 greyish white, discrete, flat, oval or round, with irregular borders. The 

 surface colonies are larger than those in the depths. The deep 

 colonies are round to oval in shape, and homogeneous. 



In gelatine stab cultures the growth has a nail-like appearance. 

 The surface growth is flat and white. In the depth the growth is 

 greyish white and beaded. Shortly after inoculation of the tube 

 many round colonies appear in the needle track, and these eventually 

 unite to form a granular white line with a finely beaded border. 



Agar Cultures. — On agar plates the surface colonies are greyish - 

 white with round or irregular borders ; in the depth the colonies are 

 greyish white and round to oval. 



Stab cultures are nail-like, and in the depth, the edges of the 

 spike are beaded. The spike of the nail has many irregular, thin 

 scale-like projections along it. 



In streak cultures, on agar and gelatine, the growth spreads over 

 the surface. 



Broth. — In broth a white sedimeiit falls to the bottom of the tube 

 and the reaction is not altered. The culture has a peculiar porky 

 odour. 



Potatoes. — On alkaline potato the growth has a straw-yellow to 

 light-brown colour, and it is abundant. On acid potato the growth 

 is scanty and white. 



Milk. — Milk is not coagulated and the re-action remains alkaline. 



In cultures the growth, if viewed by transmitted light, has an 

 opalescent appearance. 



The germ does not foi-m spores, yet notwithstanding this, it may 

 maintain its existence for a long period in the soil. 



Staining. — It is readily stained by aqueous and alcoholic solutions 

 of the aniline dyes, but it does not retain the stain when treated by 

 Gram's method. The bacillus of swine erysipelas retains the stain 

 by Gram's method. 



Organs, Secretions and Excretions in which the Virus 

 IS Contained. 



The virus is contained in the bronchial mucus, urine, bowel 

 evacuations, desquamated particles of skin, lymphatic glands, liver, 

 kidneys, spleen, and blood. 



It is eliminated from the system by the bronchial mucus, urine, 

 bowel evacuations, and skin desquamations. 



Inoculation Experiments. 

 On 20th March, at 8 p.m., a pigeon was inoculated with a large 

 quantity of juice expressed from the mesenteric glands of a pig dead 

 of the disease, and at 7 a.m. on 21st it was in a moribund condition 



