Apr. 8, i9i8 Sources of Hog-Cholera Injection 105 



of virus blood on June 25. This pig showed a fever temperature on 

 June 28, was off feed on June 29, showed weakness and conjunctivitis 

 on June 30, and died on July 11, showing well-marked hemorrhagic 

 lesions at autopsy. The secretions and excreta were collected on the 

 third, fifth, and seventh days after the virus injection — that is, on June 

 28, June 30, and July 2, 191 7. 



The eye and nose secretions were collected on sterile cotton swabs. 

 The urine and feces were collected in wide-mouth sterile bottles, the 

 excreta being collected as they were passed by the pig. The eye and 

 nose swab dilutions were prepared by washing off the cotton swabs 

 contaminated with the secretions in 60 c. c. of normal salt solution. 

 The urine was diluted with an equal volume of salt solution. The fecal 

 suspension used on the third and fifth days was prepared by shaking 

 up approximately 100 gm. of feces in 300 c. c. of salt solution. On 

 the seventh day 50 gm. of feces to 300 c. c. of salt solution was used. 

 The secretions and excreta were tested on susceptible pigs within a few 

 hours after they were collected. 



On each of the days on which the secretions and excreta were col- 

 lected, two susceptible pigs were injected with 5 c. c. each of an eye- 

 swab dilution, two with 5 c. c. each of a nose-swab dilution, two with 

 5 c. c. each of urine, and two with 10 c. c. each of fecal suspension. 



On the first day on which the secretions and excreta were collected, 

 two pigs were fed with 25 c. c. each of eye-swab dilution, two with 

 25 c. c. each of nose-swab dilution, two with 5 c. c. each of urine, and 

 two with 10 c. c. each of the fecal suspension. The materials were 

 mixed with slop when fed. The same pigs were fed with the same doses 

 on each of the succeeding days on which materials were collected. 

 These pigs therefore received three feedings of supposedly infectious 

 materials. 



On each of the three days on which the secretions and excreta were 

 collected 25 c. c. of a freshly prepared eye-swab dilution were scattered 

 on the floor of a pen containing two susceptible pigs, while 25 c. c. of 

 a freshly prepared nose-swab dilution were scattered in a second pen 

 containing two pigs. In a third pen containing tw^o susceptible pigs 

 freshly collected urine was scattered as follows: On the third day 50 

 c. c, on the fifth day 140 c. c, and on the seventh day 130 c. c. In a 

 fourth pen containing two pigs about 360 c. c. of freshly prepared fecal 

 suspension were scattered on the third, fifth, and seventh days. The 

 same pigs were thus exposed in a similar manner to the supposedly 

 infectious materials on each of the days upon which these materials 

 were collected. 



The results obtained in this experiment were briefly as follows : 



The eye and nose secretions and the feces collected on the third day 

 were infectious when injected. The urine collected on this day was not 

 infectious when injected. 



