Apr. 8, rgiS 



Sources of Hog-Cholera Infection 



107 



Table III. — Results of Experiment II on the infectiousness of blood excreta, and secre- 

 tions of infected pigs — Continued 



MATERIALS COLLECTED AND FED ON JUNE 28, JUNE 30, AND JULY 2, 1917 (THIRD, 

 FIFTH, AND SEVENTH DAYS) 



MATERIALS COLLECTED AND SCATTERED IN PENS ON JUNE 28, TUNE 30, AND JULY 2, 

 191 7 (third, FIFTH, AND SEVENTH DAYS) 



25 c. c. of eye-swab dilution. . . 



....do 



25 c. c. of nose-swab dilution. . . . 



....do 



50 c. c, 140 c. c, and 130 

 c. c. of urine. 



....do 



360 c. c. of fecal suspension. . . . 

 ....do 



Remained well. 

 Do. 

 Do. 

 Do. 

 Do. 



Do. 

 Do. 

 Do. 



These pigs were subsequently exposed to hog cholera by injections of virous blood and all proved to 

 be susceptible. 



Experiment III. — In this experiment, which was begun on August 2, 

 1 91 7, the blood, eye and nose secretions, urine, and feces were collected 

 from a cholera-infected pig on the seventh day after injection. Pig 

 2226, which furnished the blood, secretions, and excreta, was injected 

 with virus on July 26, 191 7. This pig went off feed on July 30, showed 

 conjunctivitis, diarrhea, and weakness on August 2, and died on August 

 3, exhibiting extensive hemorrhagic lesions at autopsy. 



The blood was obtained by bleeding from the tail, and was defibrinated 

 and strained through sterile gauze. The secretions and excreta were 

 collected in the same manner as in Experiment II. The blood, secre- 

 tions, and excreta were tested when fresh — that is, within a short time 

 after they were collected, and were also tested after being held for 24 

 and 48 hours at room temperature (72° to 85° F.). 



In collecting the eye secretions, three swabs were taken from the eyes. 

 One of these, soon after collection, was rubbed up in 35 c. c. of normal 

 salt solution and two pigs were injected with 15 c. c. each of the suspen- 

 sion. The two other swabs were held at room temperature. At the 

 end of 24 hours a second swab was rubbed up in 35 c. c. of salt solution 

 and two pigs injected with 15 c. c. each of the resulting suspension. At 



