io8 



Journal of Agricultural Research 



Vol. XIII, No. 3 



the end of 48 hours the third swab was rubbed up in 35 c. c. of salt solution 

 and two pigs injected, as before, with 15 c. c. each. 



Three nose swabs were prepared and tested in the same manner as the 

 eye swabs. The urine was used undiluted. In testing the feces, which 

 were diarrheal in character, about 5.5 gm. of the feces were shaken up 

 in 40 c. c. of normal salt solution and filtered through sterile gauze; 

 susceptible pigs were given 15 c. c. each of this suspension. 



After standing at room temperature for 24 hours, the blood had 

 darkened but had a normal odor. The secretions on the eye and nose 

 swabs had become dry. The urine had not changed in appearance 

 The feces had become darker in color and drier. 



After standing at room temperature for 48 hours, the blood was quite 

 dark in color and had a putrid odor. The urine showed a sediment 

 and had a stale odor. The feces were darker and had a putrid odor. 



The results obtained in this experiment were briefly as follows : 



When injected fresh, the blood, secretions, and excreta obtained on 

 the seventh day were all infectious. 



When injected after being held for 24 hours at room temperature, 

 the blood, secretions, and excreta were still infectious. 

 . When injected after being held for 48 hours at room temperature, 

 the blood, urine, and feces remained infectious, but the eye and nose 

 secretions were no longer infectious. 



The detailed results of this experiment are shown in Table IV. 



Table IV. — Results of Experiment III on the infectiousness of blood, excreta, and 



secretions of infected pigs 



MATERIALS COLLECTED AND INJECTED ON AUGUST 2, 1917 (SEVENTH DAY) 



Material injected. 



Result. 



5 c. c. of tail blood 



do 



15 c. c. of eye-swab dilution. 

 do 



15 c. c. of nose-swab dilution . . 



....do 



5 c. c. of urine 



do 



15 c. c. of fecal suspension 



do 



Died August 12. Cholera lesions. 

 Died August 16. Cholera lesions. 



Do. 

 Killed in moribund condition August 



20. Cholera lesions. 

 Died August 20. Cholera lesions. 

 Died August 19. Cholera lesions. 

 Died August 18. Cholera lesions. 

 Died August 19. Cholera lesions. 

 Died August 9. Cholera lesions. 



Do. 



