Apr. 8, 1918 



Sources of Hog-Cholera Infection 



109 



Table IV. — Results of Experiment III on the ivfectiousness of blood, excreta, and 

 secretions of infected pigs — CTontiiiued 



MATERIAIvS COLLECTED ON AUGUST 2, 1917, AND INJECTED ON AUGUST 3, 1917 (HELD 

 FOR 24 HOURS AT ROOM TEMPERATURE) 



Material injected. 



2 c. c. of tail blood. 



.do. 



15 c. c. of eye-swab dilution. 



do 



15 c. c. of nose-swab dilution . 



do 



5 c. c. of urine 



do 



15 c. c. of fecal suspension. 

 do 



Result. 



Killed in moribund condition August 



16. Cholera lesions. 

 Died August 15. Cholera lesions. 

 Died August 16. Cholera lesions. 

 Died August 19. Cholera lesions. 

 Killed in moribund condition August 



20. Cholera lesions. 

 Do. 

 Died August 16. Cholera lesions. 



Do. 

 Died August 1 1 . Cholera lesions. 

 Died August 10. Cholera lesions. 



MATERIALS COLLECTED AUGUST 2, I917, AND INJECTED ON AUGUST 4, 1917 (HELD FOR 

 48 HOURS AT ROOM TEMPERATURE) 



2 c. c. of tail blood 



....do 



15 c. c. of eye-swab dilution. 



do 



IS c. c. of nose-swab dilution. 



do 



5 c. c. of urine 



do 



15 c. c. of fecal suspension. 

 do 



Died August 12. 

 Died August 13. 

 Remained well. 



Do. 



Do. 



Do. 

 Died August 15. 



Cholera lesions. 

 Cholera lesions. 



Cholera lesions. 



Killed in moribund condition August 



IS. Cholera lesions. 

 Died August 11. Cholera lesions. 

 Do. 



a These pigs were subsequently injected with virus blood to test their immiinity, and as a result all de- 

 veloped hog cholera. 



Experiment IV. — In this experiment, which was begun on September 

 21, 1917, the eye and nose secretions, urine, and feces of a pig infected 

 by virus injection were collected on the second, third, fifth, and seventh 

 days after injection. Pig 669, which furnished the secretions and excreta 

 for this experiment, received an injection of virus blood on September 

 19. This pig showed a fever temperature on September 24, was off feed 

 on September 26, developed diarrhea and purple skin, and died on 

 October 3, showing extensive hemorrhagic lesions at autopsy. 



The eye and nose secretions were collected on sterile cotton swabs, 

 each of which was washed off in 25 c. c. of sterile normal salt solution. 

 The urine and feces were collected in the same manner as in the pre- 

 ceding experiments. The urine was used undiluted. A suspension of 

 the fecal matter was made with normal salt solution, using 5 gm. to 

 50 c. c. of the salt solution, which was then strained through sterile 

 gauze. In all instances, unless otherwise stated, the materials were 

 freshly prepared and used within a short time after they were collected. 



