no Journal of Agricultural Research voi. xiii, no. a 



On each of the days on which the above materials were collected, two 

 pigs were injected with lo c. c. each of the eye-swab dilution, two with 

 IOC c. each of the nose-swab dilution, two with 5 c. c. each of urine, and 

 two with 20 c. c. each of the fecal suspension. 



On the first day on which materials were collected, two pigs were fed 

 10 c. c. each of the eye swab dilution, two were fed with 10 c. c. each of 

 nose swab dilution, two with 5 c. c. each of urine, and two with a sus- 

 pension of 2.5 gms. of fecal matter. The materials in each instance were 

 fed in the slop. The same pigs were fed with the same doses on each of 

 the succeeding days on which the materials were collected. These pigs, 

 therefore, received four feedings of supposedly infectious materials. 



On the first day on which materials were collected 20 c. c. of eye-swab 

 dilution were scattered over the floor of a pen containing two pigs, 

 20 c. c. of nose-swab dilution were scattered in a second pen containing 

 two pigs, 200 c. c. of urine were scattered in a third pen containing two 

 pigs, and 30 gm. of feces suspended in salt solution were scattered 

 in a fourth pen containing two pigs. The same pigs were exposed in a 

 similar manner to similar amounts of supposedly infectious materials on 

 each of the succeeding days upon which these materials were collected. 

 This set of pigs was exposed, therefore, on four days to supposedly infec- 

 tious materials scattered in their pens. 



The materials collected on the fifth day were also held at room tem- 

 perature, varying from 60° to 80° F., for 24 hours, and injected in the 

 same doses as when injected fresh. 



The materials collected on the seventh day were held at room tem- 

 perature, varying from 60° to 75° F., for 24 and 48 hours, and injected 

 in the same doses as when injected fresh. The eye- and nose-swab dilu- 

 tions at the end of 48 hours remained neutral, the urine became slightly 

 more acid, and the feces, which were neutral when collected, became 

 slightly acid. 



The results obtained in this experiment were briefly as follows: 



The freshly collected secretions and excreta obtained on the second 

 day proved to be noninfectious when injected. 



The freshly collected secretions and excreta obtained on the third day 

 were all infectious when injected, except the urine, which proved to be 

 noninfectious. 



The freshly collected secretions and excreta obtained on the fifth and 

 seventh days were all infectious when injected. 



The freshly collected secretions and excreta proved to be noninfectious 

 when fed, with the possible exception of one pig fed with eye-swab 

 dilution. This pig developed hog cholera 23 days after the date of 

 last feeding. 



The freshly collected secretions and excreta also proved to be non- 

 infectious when scattered in the pens, with the exception of one pen 



