Apr. 8, 1918 



Sources of Hog-Cholera Infection 



113 



Table V. — Results of Experiment IV on the infectiousness of blood, excreta, and secre- 

 tions of infected pigs — Continued 



MATERIALS COLLECTED AND PED ON SEPTEMBER 21, 22, 24, AND 26, 1917 (SECOND, 

 THIRD, FIFTH, AND SEVENTH DAYS) 



Material injected. 



10 c. c. of eye-swab dilution . 

 ....do 



10 c. c. of nose-swab dilution. 

 ....do 



5 c. c. urine 



....do 



2 . 5 gm . of feces in salt solution . 

 ....do 



Result. 



Contracted hog cholera from contact 



with pig 746. 

 Sickened October 19 and died Oc- 

 tober 29. Cholera lesions. 

 Remained well. 



Do. 



Do. 



Do. 



Do. 



Do. 



MATERIALS COLLECTED AND SCATTERED IN PENS ON SEPTEMBER 21, 22, 

 1917 (second, third, fifth, AND SEVENTH DAYS) 



24, AND 26, 



20 c. c. of eye-swab dilution . . 



...do 



20 c. c. of nose-swab dilution. . 



....do 



200 c. c. of urine 



....do 



30 gm. of feces in salt solution . 

 ....do 



Remained well. 



Do. 

 Sickened October 12 and died Oc- 

 tober 27. Cholera lesions. 

 Remained well. 



Do. 



Do. 



Do. 



Do. 



o These pigs were subsequently exposed to hog cholera by virus injections to test their immunity and 

 all developed cholera except pig No. 758, which remained well and probably possessed considerable 

 natural immunity. 



In the four experiments which have just been described the virus 

 was found to be present in the circulating blood of the cholera-infected pig 

 on the first, second, third, fifth, seventh, and ninth days after injection. 



The experiments indicate that the urine may be infectious as early 

 as the first day after injection. In one experiment the urine was found 

 to be infectious from the first to the ninth days, while in two experiments 

 it was not infectious on the third day but was infectious on the fifth and 

 seventh days. F'rom these results it appears that, while the virus may be 

 present in the urine earlier than the fourth day, it is quite regularly 

 present in the urine of cholera-infected pigs by the fourth or fifth days. 



In one experiment the virus was present in the feces on the second 

 day, while in another experiment it was absent on the second day. In 

 three experiments the virus was present in the feces on the third day, and 

 it would thus appear that virus is thrown off quite regularly in the feces 

 by the second or third day. 



In two experiments the virus was absent from the eye and nose secre- 

 tions on the second day, while in three experiments it was present in 

 these secretions on the third day, and it is worthy of note that on those 

 days there was no abnormal discharge from either the eyes or nose. It 



