114 



Journal of Agricultural Research 



Vol. XIII, No. 2 



would thus appear that the virus is present quite regularly in the eye 

 and nose secretions by the third day. 



The foregoing results are summarized in Table VI. 



Table VI. — Summary of results obtained in Experiments I to IV from injection tests 

 of blood, excreta, and secretions of cholera-infected pigs<^ 



EXPERIMENT 1 



EXPEIUMENT II 



EXPERIMENT in 



+ 



+ 



+ 



+ 



EXPERIMENT IV 



o + indicates that the material was virulent: the injected pigs sickened or died. — Indicates that the 

 material was not virulent: the injected pigs remained well, and contracted cholera upon subsequent ex- 

 posure. 



In contrast with the foregoing results, which were ootained by injecting 

 the various materials, the results obtained from feeding the same mate- 

 rials and from scattering the same in pens with susceptible pigs were 

 strikingly different. When fed and when scattered in pens, the freshly 

 collected secretions and excreta obtained on the second, third, fifth, and 

 seventh days proved to be noninfectious, with the possible exception of 

 two pigs. One pig which was fed with eye secretion developed cholera 

 23 days after the date of the last feeding, and one pig exposed to nose secre- 

 tion scattered in the pen developed hog cholera 16 days after the material 

 was last scattered in the pen. It thus appears that although the virus 

 is undoubtedly thrown off in the secretions and excreta of the sick animal 

 at an early stage of the disease and the disease may be surely and readily 



