Apr. 8, 1918 Sources of Hog-Cholrea Infection 119 



In this experiment the disease was transmitted by contact as late as 

 the tenth day after infection, or the seventh day after the appearance 

 of the first visible symptoms. 



Experiment VII. — Pig 932 was injected with 5 c. c. of virus blood 

 on October 25, showed first visible sickness on October 30, and devel- 

 oped the usual cholera symptoms. This pig was transferred to a clean, 

 disinfected pen on November 11, 17 days after inoculation and 12 days 

 after the first appearance of visible symptoms, and two susceptible pigs, 

 No. 1 140 and 1141, were placed in the same pen. At the time of trans- 

 fer, pig 932 was off feed and showed a fever temperature, conjunctivitis, 

 weakness, and diarrhea. 



The two exposed pigs. No. 1140 and 1141, contracted cholera from 

 contact with the sick pig and showed first visible symptoms on Novem- 

 ber 20, 9 days after exposure. Pig 1 140 was killed when in a moribund 

 condition, on November 25, showing extensive hemorrhagic lesions at 

 autopsy. Pig 1141 died November 25 and exhibited extensive hemor- 

 rhagic lesions. Hog 932, which furnished the exposure, was found dead 

 on November 16 and showed characteristic hemorrhagic lesions of 

 cholera. 



In this experiment the disease was transmitted by contact as late as 

 the seventeenth day after inoculation, or the twelfth day after the appear- 

 ance of first visible symptoms. 



Experiment VIII. — Pig 794 was injected with 5 c. c. of virus blood 

 on June 30. The animal w^as first visibly sick on July 7 and developed 

 characteristic cholera symptoms. On July 21, 21 days after inocula- 

 tion and 14 days after the appearance of visible symptoms, this pig 

 was first scrubbed with soap and water, next with compound cresol 

 solution, again with soap and water, and was transferred to a clean, 

 disinfected pen. A susceptible pig, No. 811, was placed in the same 

 pen. At the time of transfer, pig 794 showed a temperature of 103.8'^ 

 and was recorded as off feed and as showing conjunctivitis, diarrhea, 

 and red skin. 



The exposed pig. No. Six, contracted hog cholera from contact with 

 the sick pig, showing the first visible symptoms on July 25, four days 

 after exposure; this pig died on July 30, and the autopsy revealed hem- 

 orrhagic lesions. Pig 794, which furnished the exposure, died on August 

 4 and showed hemorrhagic lesions and ulceration of cecum and colon. 



In this experiment the disease was transmitted by contact as late as 

 the twenty-first day after inoculation, or the fourteenth day after the 

 appearance of first visible symptoms. 



Experiment IX. — Pig 904 contracted hog cholera by association with 

 sick pigs and developed characteristic cholera symptoms, including weak- 

 ness, loss of appetite, conjunctivitis, and diarrhea. This pig was trans- 

 ferred to a clean, disinfected pen on October 28, 2 1 days after the appear- 

 41812°— 18 4 



