Apr. 15. 1918 A Leajhlight of Kalmia latijolia 205 



medium as a flat, furry layer. At the end of about 7 days a dark green- 

 ish tinge appears about the center or at the margins of the com meal, 

 followed by minute dark -green to black points, — the beginnings of the 

 pycnidia. In about 15 to 20 days after inoculation the entire surface, 

 sides, and often the bottom of the com meal are covered with velvety, 

 irregularly shaped, large fruiting bodies frequently having dark -green 

 to black caps, and pure-white to light-gray and olive-green sides (PI. 

 16, A, B). Sometimes when the pycnidia are very small (within 13 to 

 15 days after inoculation of the flask), the surface and sides of the 

 medium will become yellow from the extmsion of the waxy coils and 

 roundish masses of spores. At other times bodies are produced that 

 are identical externally with the pycnidia, but which form no spores 

 at all, even when held for months under various conditions. The spore 

 masses vary in color, but are usually waxy yellow through amber to 

 cream. 



A greater abundance of mycelium is produced on steamed potato 

 cylinders than on any other medium. Pycnidia frequently form within 

 five days after inoculation, and vary in color from deep mouse-gray to 

 dark ivy-green. When old, the seal-brown to black stroma is sharply 

 marked with wavy white bands, and is tough and leathery in texture. 



On corn-meal agar very little mycelium is produced. The pycnidia 

 are rather small and often clustered, but form rapidly. Spores are 

 produced promptly within the scattered groups of pycnidia, and but 

 very few sterile bodies are formed. 



Synthetic agar is a very good medium for the isolation of the fungus, 

 as the mycelium grows quickly and abundantly, the medium at the same 

 time inhibiting or preventing the growth of most intmding bacteria. 

 The colonies are very beautiful, feathery in appearance, and of a color 

 between pale dull gray and white. No pycnidia or sclerotia have ever 

 been observed on this medium. 



When litmus milk was added to steamed corn-meal flasks, the sub- 

 stratum was blued very decidedly at first, but at the end of 10 days was 

 bleached almost throughout (cream), the control flasks remaining lilac. 



In litmus milk a fair growth occurred, with coagulation of the milk at 

 the end of five days, but the casein was not precipitated until the 

 seventh day. The litmus was slightly reduced after 15 days, and at the 

 end of 30 days was entirely reduced and the milk proteolyzed. In one 

 tube proteolysis occurred in 13 days. 



The growth of the fungus in Cohn's solution demonstrates its ability 

 to obtain nitrogen from ammonium salts. There was no liquefaction 

 of the medium when the fungus was grown in beef gelatin, and no pycnidia 

 were formed. 



