354 Journal of Agricultural Research voi. xnr, no. 7 



ORGANOLEPTIC TESTS 



The appearance of the oil as determined by the unaided eye varied 

 with the depth of stratum and character of the light from transparent 

 olive-green to opaque, almost black. All efforts at color differentiation 

 without an instrument proved unsatisfactory, but were continued 

 throughout the experiment, as a tintometer was not available for the 

 first four years. In amounts of 6 ounces, the basis employed, the oil 

 will be designated a dark olive-green. The green greatly exceeded that 

 in most olive oils offered in local markets, probably due, as the manu- 

 facturer claims, to differences in soil and climatic conditions, together 

 with possibly small variations in manufacturing methods. The oil had 

 an excellent body and a pronounced olive odor. 



PHYSICAL TESTS 



20° 

 Specific gravity rro C o. 9130S 



Specific gravity — 5 C. (calculated) o. 911 52 



Specific gravity 25° C. (U. S. P. standard) o. 910- o. 915 



20° C. 

 Refractive index n — j) — (Abbe) i. 4687 



Viscosity 70° F. (Redwood) 12. o 



Valenta test (B. and A. 99.5 per cent acid) 87. 5° C. 



Elaidin test Green, semisolid 



CHEMICAL TESTS 



Saponification number 190. 636 



Saponification number (U. S. P. standard) 190-195 



Acid number (a) i. 990 



Ether number (e) 188. 646 



Total fatty acids ( 1.00-0.0002 2 5 94e) 95-74 per cent 



Neutralization number (n) 199. 12 



Mean molecular weight 281. 78 



Free fatty acids as oleic acid and as — i. 00 per cent 



Glycerol (o.ooo54703e) 10. 32 per cent 



Reichert-Meissl number None 



Polenske number o. 13 



Insoluble acids 95-4° per cent 



lodin number (Wijs) 83. 45 



lodin number (U. S. P. standard) 79- 90 



Acetyl number 5. 69 



COLOR TESTS 



Baudouin test for sesame oil Nil 



Bechi silver nitrate test for cottonseed oil Nil 



Halphen test for cottonseed oil Nil 



Nitric- acid test for seed oils Brown, slight coagulation 



All organoleptic, physical, chemical, and color tests indicated a pure 

 olive oil, with the exception of the nitric-acid test which may be dis- 

 regarded, as it is no longer designated by the Pharmacopoeia. 



