37^ Journal of Agricultural Research voi. xiii, no. 7 



Bouillon over chloroform. — Gro^nh occurs, but is retarded. Only a slight 

 clouding occurs in 7 to 8 days, and in 30 days the growth is still slight. 



Uschinsky's solution. — The organism does not grow readily in this medium. 

 There is a slight growth in 4 days. In 30 days it is still slight, with only a faint white 

 clouding. This was repeated seven times. No growth occurred in three of the tests. 



Cohn's solution. — The growth is very faint, and often does not occur. Out of 

 eight tests definite growth occurred three times, faint growth twice, questionable 

 twice, and once not at all. One infectious colony of the two used throughout this 

 work might grow in one lot of media, while the other might not. 



Fermi's solution. — No gro^^-th occurred. 



Sterile milk. — In 5 days the medium is clear to a depth of only 3 mm. below the 

 surface. In 7 to 9 days it is about half clear, with no acid coagulation, but with a 

 heavy curdlike precipitate. There is a slow separation of curd and whey by 11 days. 

 At 16 days there is no yellow color in the whey or the precipitate, but the bacterial 

 growth at the surface is yellow. In 49 days very little curd is left, and this is present 

 in little balls. The color of the whey has changed to yellow again, a light-orange 

 yellow. 



Litmus milk. — There is a slow reduction. The color changes in rings, a deep 

 blue at the top, shading down to lilac litmus color at the bottom. In 5 days one-third 

 of the medium from the top down is clear, and is darker blue, with a yellow bacterial 

 precipitate. In 7 days a curd has formed; there are still three shades in the medium, 

 anthracene-purple at the top, shading to a brownish at the bottom. At 18 days the 

 purple color has disappeared, and the entire medium is a light brown; the curd is in 

 suspension. After a month the medium is light brown throughout, except at the 

 very surface, where it is purple. There is a viscid mixture of curd and bacteria 

 through half the medium, numerous balls of white curd floating in this viscid mixture. 



Nutrient gelatin. — The colonies are slow in appearing on peptone gelatin (+10) 

 plates at 12° to 15° C. In two tests made the colonies did not appear before 7 to 9 

 days. Even when the plates are not thickly sown, the colonies do not develop a 

 diameter larger than 4 mm. They are yellow, round, shining, and thicker than beef- 

 agar colonies. Buried colonies are both round and oval. Liquefaction begins when 

 the colonies are 3 days old, in little cups around them, continuing slowly. When they 

 are 16 days old, the gelatin of the thinly sown plates has not entirely liquefied. 



The stab cultures liquefy slowly also at a temperature of 12° to 15° C. In 2 days 

 there is slight growth on the surface and along the line of puncture, but no liquefac- 

 tion. In 9 days there is a slight crateriform liquefaction, and in 12 days the lique- 

 faction has reached almost across the surface of the gelatin. In 30 days 1.5 cm. of the 

 medium are liquefied, in 40 days one-half, and in 57 days all except one-sixth at the 

 bottom of the tube. 



Steamed potato cylinders. — There is abundant growth in 2 days at a tempera- 

 ture of 25° C. The growth is smooth, thick, viscid, shining; the color is empire- 

 yellow (PL E). In 14 days the gro^vth is a dark olive-buff, and the medium has 

 changed to a grayish brown. 



There is a feeble diastasic action on the starch. 



OTHER CULTUR-\L FEATURES OF THE ORGANISM 



Indol. — There is slight production of indol in i per cent peptone-water cultures 10 

 days old. It is still slight when the cultures are 16 to 20 days old. 



Nitrates. — Nitrates are not reduced. Tests were made when nitrate bouillon 

 cultures were 7 and 17 days old. 



Ammonia production. — Moderate. 



Hydrogen sulphid. — Hydrogen sulphid is produced. Cultures of beef agar, beef 

 bouillon, milk, and potato cylinders were tested by hanging lead-acetate paper in 



