Mayi3. i9i8 SoTue BacteHal Diseases of Lettuce ' 38- 



BouiLLON CONTAINING SODIUM CHLORiD. — There is good growth at 

 once in neutral beef bouillon containing 3 per cent of sodium chlorid, a 

 slight retardation but heavy growth in that containing 5 per cent, only a 

 fair amount of growth in the bouillon containing 6 per cent, and none 

 at all in that containing 8 per cent. 



Gelatin plates. — Colonies are up in 4 days on +10 beef-peptone 

 gelatin plates at 11° to 15° C; are i mm. in diameter, a deep-cream 

 color, bluish in transmitted light, with margins slightly indented. 

 Liquefaction (cup-shaped) begins when colonies are 2 days old. No 

 color change occurs at this age. When colonies are 5 days old, they are 



2 to 4 mm. in diameter and the gelatin is greened around them for some 

 distance. This color is mineral -green. At 15 days the liquefaction is 

 still cup-shaped around colonies, but the green color has spread through 

 the entire gelatin. At 25 days the gelatin is not all liquefied. 



Gelatin stab. — In beef -peptone gelatin -l-io stabs Hquef action 

 begins in from 2 to 4 days in a crateriform way at a temperature of 11 ^ 

 to 15° C. Growth is good at the surface, feeble along the stab; there is 

 a faint green color at the surface. In 6 days gelatin is liquefied three- 

 fourths across the surface of the stab, the color just below being viridine 

 green. In 15 days the surface of the gelatin is liquefied straight across 

 for a depth of i cm. and the color, which extends halfway down the 

 tube, is yellow-green, a brighter green than that of the plates. 



Uschinsky's SOLUTION. — There is heavy clouding in 24 hours. In 



3 days the upper half of the medium is greened. In 5 days there is a 

 heavy pellicle, and the medium throughout has become Veronese-green. 



Corn's solution. — The organism does not grow in Cohn's solution. 



Fermi's solution. — No growth. 



Sterile milk. — In 3 days about one-third of the medium is cleared 

 and is a sea-foam-green color; the rest is a soft curd. In 20 days the milk 

 is all cleared Avith curd in suspension. The color is citron-green. In 47 

 days the milk is a darker color, lime-green. The curd is in suspension in 

 bottom of tube ; the rest of the liquid is clear. 



Litmus Milk. — In 2 days the color of the medium has begun to change 

 in rings, a turbid reddish color at the top for 4 mm., then 2 cm. of a lighter 

 shade below, and next the ring of lilac-litmus color. A pellicle is on the 

 surface; there is no coagulation. In 4 days none of the original lilac- 

 litmus color is left; the medium is clear and half of it is reddish brown 

 (dark vinaceous drab) ; the rest is a lighter color. At 7 days the upper 

 half of the medium has more red color in it — is dark mirieral-red. The 

 rest of the milk is a reddish-tan color. There is a heavy pellicle and a soft 

 curd. In 25 days the medium has changed to a dark-blue shade, is blue- 

 violet-black. 



Steamed potato cylinders. — There is a thin watery growth covering 

 the surface of the cylinder in one day. Plate E, 5> gives the appear- 

 ance at the end of the second day. In 4 days this growth is a pinkish- 



