juneio. i9i8 Experiments with Coccomyces spp. from Stone Fruits 547 



veniently mounted on a small truck which will easily pass through 

 standard doorways, will probably be much more satisfactory. A supply 

 of compressed air with which to charge such apparatus is highly desirable, 

 since charging it by means of an automobile pump is as laborious and 

 time-consuming as atomizing by hand. 



Inocula. — The inocula were prepared in the same fashion as in the 

 experiments of 1916 (p. 541), except that in a few cases the cultures used 

 were not from single-spore strains. In all cases, however, the isolations 

 were made by the method previously described by the writer,* their 

 purity being established by frequent microscopic examinations in their 



$c/pp/ypipe 



Fig. I. — Moist chamber used in the outdoor inoculation experiments of 1917. 



early stages of development and by later cultural tests. Inasmuch as 

 no differences were observed between the results from single- and mul- 

 tiple-spore cultures, the strains are not distinguished in this regard in the 

 tables. 



Method oif inoculation. — The plants were inoculated by the method 

 used in 191 6 (p. 541). The moist compartments, however, were left in 

 place only one day. 



Before each inoculation, in order to facihtate the taking of records, 

 markers of white string were tied near the apexes of several twigs on each 

 plant. In this way the results of successive inoculations on the same 



■ Keitt, G. W. simple technique for isolating einglE-sporb strains of certain types op fungi. 

 In Phytopathology, v. s, no. 5, p. 266-269, i fig- i9iS- 



