622 



Journal of Agricultural Research 



Vol. XIII, No. 12 



Table I. — Effects of various chemicals on the infectivity of the virus of the mosaic 

 disease of tobacco — Continued 



SODIUM CARBONATE 



Concentration. 



Solution pre- 

 pared. 



Inoculation 

 made. 



Plants used. 



Results. 



I gm. to 50 c. c. of virus solution 



1 gm. to 100 c. c. of virus solution. . 

 1 gm. to 200 c. c. of virus solution. . 

 1 gm. to 300 c. c. of virus solution. . , 

 1 gm. to 400 c. c. of virus solution. . , 

 I gm. to 500 c. c. of virus solution. . , 

 I gm. to 600 c. c. of virus solution. . . 

 I gm. to 800 c. c. of virus solution. . , 

 I gm. to 1,000 c. c. of virus solution 

 I gm. to 1,200 c. c. of virus solution 

 I gm. to 1,500 c. c. of virus solution, 



Virus without addition 



Tap water only (control) 



Nov. 30, 1915" 



.do. 

 .do. 

 .do. 

 .do. 

 .do. 

 .do. 

 .do. 

 .do. 

 .do. 

 .do. 

 .do. 

 .do. 



Dec. 17, 1915 (17 



days later). 

 do 



.do. 

 .do. 

 .do. 

 .do. 

 .do. 

 .do. 

 .do. 

 .do. 

 .do. 

 .do. 

 .do. 



10 Connecticut 3 plants mosaic. 

 Broadleaf. 

 .do 4 plants mosaic. 



.do. 

 .do. 

 .do. 

 .do. 

 .do. 

 .do. 

 .do. 

 .do. 

 .do. 

 .do. 

 .do. 



6 plants mosaic. 

 9 plants mosaic. 



Do. 



8 plants mosaic. 



9 plants mosaic. 



8 plants mosaic. 



9 plants mosaic. 



7 plants mosaic. 



8 plants mosaic. 



Do. 

 All healthy. 



SODIUM HYDROXID 



I gm. to 50 c. c. of virus solution 



I gm. to 100 c. c. of virus solution. . 

 I gm . to 200 c. c. of virus solution . . 

 I gm. to 400 c. c. of virus solution. . 

 I gm. to 800 c. c. of virus solution. . 

 I gm. to 1,000 c. c. of virus solution 

 I gm. to 2,000 c. c. of virus solution 

 I gm. to 4,000 c. c. of virus solution 

 I gm. to 8,oco c. c. of virus solution 



Virus without addition 



Tap water only (control) 



Apr. 25,1914 



.do. 

 .do. 

 .do. 

 .do. 

 .do. 

 .do. 

 .do. 

 .do. 

 .do. 

 .do. 



Apr. 27, 1914 (2 



days later). 

 do 



....do 



....do 



do 



do 



do 



do 



do 



do 



do 



10 Maryland 

 Mammoth. 



....do 



....do 



....do 



....do 



....do 



....do 



....do 



....do 



....do 



do 



All healthy. 



Do. 



Do. 



Do. 



Do. 



Do. 

 I plant mosaic. 

 10 plants mosaic. 



Do. 



Do. 

 All healthy. 



MANGANESE SULPHATE 



I gm. to 12. 5 c. c. of virus solution. 



1 gm. to 2S c. c. of virus solution. . . 

 I gm. to so c. c. of virus solution . . . 

 I gm. to 100 c. c. of virus solution.. 

 I gm. to 200 c. c. of virus solution.. 

 I gm. to 500 c. c. of virus solution. . 

 Tap water only (control) 



Oct. 13,1915a 



.do. 

 .do. 

 .do. 

 .do. 

 .do. 

 .do. 



Dec. 28, 1915 (76 

 days later). 



....do 



....do 



do 



do 



do 



do 



7 plants mosaic. 



8 plants mosaic. 



9 plants mosaic. 



10 plants mosaic. 



Do. 

 9 plants mosaic. 

 All healthy. 



SODIUM CHLORID 



I gm. to 25 c. c. of virus solution. . 



I gm. to so c. c. of virus solution. . 

 I gm. to 100 c. c. of virus solution. 

 I gm. to 200 c. c. of virus solution. 

 I gm. to 400 c. c. of virus solution. 

 Tap water only (control) 



Jan. 24, 1916 



.do. 

 .do. 

 .do. 

 .do. 

 .do. 



Mar. 5, 1916 (41 

 days later). 



do 



do 



do 



do 



do 



All healthy. 



Do. 

 7 plants mosaic. 



Do. 



10 ] s plants mosaic. 



10 All healthy. 



ALUMINIUM SULPHATE 



I gm. to 100 c. c. of virus solution. . . 



Feb. 19, 1916 



I gm. to 200 c. c. of virus solution do. 



I gm. to 400 c. c. of virus solution do. 



Virus without addition do . 



Tap water only (control) do. 



Mar. 7, 1916 (17 

 days later). 



do 



do 



do 



do 



3 plants mosaic. 



10 plants mosaic. 

 8 plants mosaic. 

 6 plants mosaic. 

 All healthy. 



a Virus filtered clear through filter paper before solutions were made. 



