APPENDIX F 103 



Thus day by day the weight of each bird was taken, and its caecal excreta, 

 collected from the clean board floor, were examined without the discovery of 

 a single Trichostrongylus ovum. 



Even on November 5th, when the excreta were again examined, neither 

 of the birds had become infected. This was a month after exposure to what 

 was then believed to be infection. 



M. might have been infected by the heather washings. 



P. might have been infected by the larval Trichostrongylus. 



S. was a control, and would have remained unaffected. 



It was at this point that Dr Leiper suggested a new plan for experimental 

 infection, but it was not put at once into execution, as the difiiculties of keeping 

 cultures of Trichostrongylus larvae alive were not yet completely over- 



New 



come. Almost every culture died off before complete metamorphosis method of 

 had taken place, chiefly on account of the difiiculty of feeding the 

 larvae, and yet avoiding decomposition in the food. In pure water they always 

 died. 



The following experiments were therefore made later. 



Briefly, they can be summarised as follows, the notes referring to weight, 

 dosage, and temperatures being given in greater detail below. 



Of three adult Grouse purposely infected with Strongylosis, two died of 

 marked Strongylosis. One, still living in May 1910, proved very resistant, 

 although infected slightly. 



One adult Cxrouse accidentally infected with Trichostrongylus died with 

 marked signs of Strongylosis. 



Of six young Grouse purposely infected with Trichostrongylus, two 

 were killed accidentally, one of which gave no result, but the other was 

 distinctly infected with Strongylosis. One died of marked Strongylosis. One 

 died markedly infected with Strongylosis. Two are still living. May 1910. 



Of four young Grouse purposely infected with coccidia, one died and 

 three were killed, all four showing Coccidiosis. 



Of three young Grouse accidentally infected with coccidia, all died showing 

 marked signs of Coccidiosis. 



