'M2 soil, I'UOTOZUA IX SOUTH AFIUCA 



Ifhi Scliool ot Agiiciiltute, Middelburg, and from the George 

 disl)ic't. 



Rosebank Experiment Station. — Three areas were examined, 

 'riic lirst saiiipk' consisted of twelve one-inch hiyers of soil, taken 

 very carefully, without either admixture or wastage, and forming 

 a compact block twelve inches deep. A thirteenth sample was a 

 composite one of all the layers, one to twelve inches inclusive. 

 The samples were secured on May 15, 1922, rain having fallen 

 the previous day. (This was at the commencement of the Cape 

 i'eninsula rainy season). They were Liesbeek alluvial soil, taken 

 from cultivated land within three yards from the river, and the 

 soil had not been disturbed. These samples are referred to as 

 Liesbeek 1, 2, B 18. 



Soil from the second area, termed Liesbeek 14, was a com- 

 posite sample froin a large open storm water drain under the 

 fig trees and was detritus from the streets and gardens of Rose 

 bank. 



Soil from the third area, termed Liesbeek 15, was from a sod 

 in a grazing camp at Kosebank, that had not been disturbed for 

 years. 



Cultures were made in each case in the proi)ortion of 5 grams 

 of soil to 10 c.c. of boiled sterile tap water. 



In no case were trophic Protozoa detected on direct 

 examination. The first motile Protozoa were found in the culture 

 fluid 25 hours after the culture w\iis made. Each specimen of 

 soil gave a feebly acid reaction to litmus. This series of samples 

 also yielded interesting information as to the occurrence of 

 Protozoa at different depths in the soil. (See p. 360.) 



The Protozoa found in each sample may now be set forth, 

 beginning with the laj'er from the surface to one inch deep, termed 

 Jjiesbeek 1 . 



Liesbeek 1. — The Protozoa found in water culture were: — 

 Phizopoda. — Anioeha proteus, A. Umax, A. guttula, 



Euglyphd sp. 

 Mastigophora. — Fcranoiui iric}i.op]iofu)u , MastUjaiua'ba sp., 



Oikomonas tcrmo, Bodo (Protcazekia) parva. 

 Infusoria. — Prorodon ovit))i, Lacriimaria olur, Cyclidiu iii 



gla iicoDia , VorticcUn cdin panida . 



Liesbeek 2. — The Protozoa foimd in water culture were :— 



Ehizopoda. — A^ioeba Umax, A. guttula, EugJgpha sp. 

 Mastigophora. — Peranema tricho phorum ^ Oikomonas tcr)no, 



Bodo (P.) parva, Euglciia oxjiuris, Pleuroxionas 



j'acithnis. 

 Infusoria. — Holoplirga ovum, hacrgmaria (dor. Cip-Jidium. 



glaucoma, Paramopciuni aurclia, P. pudrinu.))} , ^^orti- 



cclht campauuJa. 



Liesbeek 3. — The following l^rotozoa were found in water 

 cidtiu'e : — • 



Pihizopoda. — Anmha protciis, A. Umax, Euglgpha sp. 



