SOIL PKOTOZOA IX SOUTH AFRICA. 34o 



On water culture the following Protozoa were obtained:- — 

 Rhizopoda. — Amoeba j)rotcus, A. Umax, A. radiosa, A. 

 verrucosa, A. gutfala, Difjiugia pijrifonnis, D. (jloha- 

 losa, Euglypha- sp. 

 Heliozoa. — Actinoplirys sol, Acanthocytitis aculeata. 

 Mastigophora. — Peranenia tvichophoruwi, Mastigainoeha 

 sp., Oikovionas ternio, Bodo (P.) parva, Eiigloia 

 oxyuris, Herpetovionas terricolce. 

 Infusoria. — Lacrynuiria olor, Paranicccintii pnfiinniii , 

 Cyclidhun glaucODia , Spirostonium ambigitnni , 

 StyloiiycJiia )nytilus, Urolcptus piscis. 

 This sample of soil yielded no less than 22 species of Pro- 

 tozoa, and they were present in relatively large numbers. 



Liesbeek 15. — This specimen consisted of a block of soil cut 

 fiom a sod in a grazing camp. The latter had not been touched 

 for years. 



On culture, extremely few organisms were obtained, and the 

 periods of negative results were much more numerous than those 

 on which Protozoa were observed. 

 The Protozoa found were: — 



Ehizopoda. — Amwha Umax, Euglypha sp. 

 Mastigophora. — Oikomonas teniw, Bodo (P.) parva. 

 Infusoria. — Holopltrya ovidu, Colpoda cucullus, Vooiicella 

 camqjanula . 

 It seems possible that years of trampling by cattle had so 

 compressed the surface of the soil as to make it an unfavourable 

 teiTain for Protozoa. 



Grootfontein, School of Agriculture, Middelburg. C.P. — 

 Two samples of Red Karroo' soils were obtained on April 11, 

 1922, namely, virgin Red Karroo and cultivated Red Kan'oo. They 

 were taken at a depth of nine inches below the surface from the 

 sites at which previous samples for examination had been ob- 

 tained. (See this Journal, vol. XVIII, pp. 979, 380.) The 

 reaction to litmus was feebly alkaline in each case. 



Water cultures yielded the following Protozoa: — 



(i) Virgin Red Karroo. 

 Rhizopoda. — E ughjpha sp. 

 Mastigophora. — Peranerna trichopliorji m , Oikomonas fcrmo, 



Pleuromonas jaculans. 

 Infusoria. — Ijacryniaria olo^r, Cyclidium glaucoma. 

 Rhizopoda. — Ainceba Umax, Euglypha sp. 

 Mastigophora. — Peranerna trichophoruni, Oikomonas termo. 

 Bodo (P.) parva, Ccrcomonas crassicauda, Pleuro- 

 (ii) Cultivated Red Karroo. 



vionas jaculans. 

 Infusoria. — Coleps liirtus, Cyclidium glaucoma. 



As in the previous examinations, there is again a marked 

 difference between the protozoal fauna of virgin and cultivated 

 Karroo soils, the latter being richer in genera and also having a 

 greater total number of Protozoa present. 



