SOIL PROTOZOA IX SOUTH AFRICA. 847 



Euglyplia and cysts of a ciliate were seen. On water culture the 

 ioUowing Protozoa were obtained: — 



Ehizopoda. — Amoeba proteus, A. guttula, Diffiugia globit- 



losa, Eaglypha alveolata, Euglypha sp. 

 Heliozoa. — Actinophnjs sol. 



Mastigophora. — Peranema tricliopliorum, Oikomonas ternio, 

 Bodo (P.) parva, Ccrco^nouas crassicauda, Entosiphoii 

 sulcatum, Euglena oxyuris. 

 Infusoria.- — Lacrymaria olor, Coipoda cue alius, Cyclidiuni 

 glaucoma, Paramoecium aurcjla, Stylouycliia DiiitiJus, 

 Vorticella companula. 

 There is a marked difference, particuhirly seen in the Infu- 

 soria, between the fauna of the two cultures of soil under forestry 

 cultivation. The part in which the growth of Pinus insignis was 

 good showed larger numbers of genera of Protozoa and also a 

 larger total number of organisms than the culture of soil on which 

 the pines had made less progress. 



Transvaal Soils. 



Observations were made on samples of soil obtained from the 

 same districts as those reported on previously by us, in order 

 to try to determine any seasonal variation in the protozoal fauna 

 of the soil in those neighbourhoods. Unfortunately, the data 

 available at jDresent hardly seem suflficient to justify very definite 

 conclusions. The records of the observations ai-e as follow: — 



Johannesburg, Houghton Estate. — The specimen of soil was 

 collected on March 23, 1922, at the end of the Transvaal rain}^ 

 season. It was garden soil under cultivation as a flower and 

 vegetable garden, and the sample was a mixed one of the top 

 nine inches of soil. Its reaction to litmus was acid. 



Water cultures yielded tlie following Protozoa: — 



Ehizopoda. — A))ia'ba protean, Diffiugia pgvifonnis, ArecUa 

 vulgaris. 



Mastigophora . — Oikomonas termo. 



Infusoria. — Euplo4es harpa . 



Potchefstroom. — Soil was collected from a cultivated fallow 

 on the farm of the Agricultural College, Potchefstroom, on August 

 1. It was a light brown sandy loam, and was a mixed sample of 

 all layers up to and including twelve inches in depth. Its 

 reaction was feebly acid to litmus. No Protozoa were observed 

 on direct examination. 



In water culture the following Protozoa were obtained, the 

 numbers always being very small and with long intervals between 

 the successive appearances : — 



Ehizopoda. — Amoeba. proteus, A. Umax, ^4. guttula, 

 Diffiugia glohuJosa, Euglypha sp. 



Heliozoa. — Aetiuophrys sol. 



Mastigophora. — Peranema trichophorum, Oikomonas ternio, 

 Bodo (P.) parva, Entosiphon sidcatum, Pleummonas 

 jaculans. 



Infusoria. — Halteria grandinella, Zh'oleptus piscis. 



