SOIL PROTOZOA IX SOUTH AFRICA. 849 



ylobulosa and Euglyplia sp. No motile organisins were detected. 

 Water cultures yielded the following Protozoa: — 



Khizopoda. — DiffiiKjia glohulosa, EiigJijpiia sp. 

 Heliozoa. — Aca)ifltocystis aculeaia. 



Mastigophora. — MastigcDnoeba sp., Oikonionas teriiw, Bodo 

 (Proivazekia) parva, Eugleiia spirogym, Ccicoinonas 

 crassicauda, PI euro mo nets jaculans. 

 Infusoria. — Holophrya ovum, Colpidium slii'tfui}!, Cycli- 

 dium glaucoma, Spirostomuvi ambigiiuni , Si iiloiiychia 

 Diytilus, VorficcUa campanula. 

 With the exception of Pleuromonas jaculans, none of the 

 Protozoa was abundant. 



(ii) Cultivated soil. This consisted of a dark chocolate loam, 

 very finely divided. A surface film of soil wetted with boiled tap 

 water was examined prior to culture. Cysts of Amcjebfe, 

 Difflugia glohulosa and Euglypha sp. were observed. No motile 

 organisms were present. On water eultui-e the following Protozoa 

 were obtained: — 



Ehizopoda. — Amo'ha Umax, Difiiugia glohulosa, Euglyplia 



Mastigophora. — Perancma irichophonon , Oikonionas 



termo, Bodo (P.) parva, Pleuromo)ias jaculans. 

 Infusoria. — Lacrymaria olor, Colpoda cucuUus, Colpldiuin 

 colpoda, CycUdium glaucoma. 

 The result of these two cultures was somewhat svu'prising, 

 the uncultivated soil yielding more genera of Protozoa than the 

 cultivated soil. Several organisms were common to both cul- 

 tures. So far as total numbers of organisms were concerned, 

 the culture of cultivated soil yielded a greater total than that 

 of the uncultivated soil. The Protozoa were also more actively 

 motile in the culture of the cultivated soil. 



Glen. — Six samples of soil were obtained from different parts 

 of the lands of the School of Agriculture, Glen, on September 

 20 during bright sunshine. They were mixed samples of tlie first 

 six inches depth of soil. 



Glen (i). This sample was collected from the river lands 

 and consisted of virgin soil on the north bank of the Modder 

 River, one hundred yards to the south-east of the distributor, 

 in the mimosa belt. This soil gave an acid reaction to^ litmus. 

 No Protozoa were detected on direct examination. Water cul- 

 tures yielded the following Protozoa: — 



Rhizopoda. — Amoeba guttula, Euglyplia sp. 

 Mastig'ophora. — Oikomonas termo. Bodo (P.) pnrva, 

 Pleuromonas jaculans. 

 Infusoria. — Holophrya ovum. Lacrymaria olor. Am nlulcptiis 

 ciignus, Colpoda cuciillus, Cgclidium glaucoma, 

 Paramoeciujn aurelia. 

 Glen (ii). This sample was from the river lands, from a 

 cultivated patch of Sudan grass, on the west of the road to the 

 station and north of the main furrow. The area is one of mixed 

 bu.sh and grass veld, used for irrigation and grazing. The 



