'S50 SOIL 1'1U)T()Z()A l.\" SOITII AKltlC'A. 



reaction of the soil to litmus was practically neutral. Water 

 cultures in the proportion of 5 grams of soil to 10 c.c. of boiled 

 sterile water -were made. No organisms were detected on direct 

 examinations. The water cultures yielded: — 

 Rhizopoda. — Euglypha sp. 



INIastigophora. • — ■ Perariema tricliophoniiH , Oikomouas 

 iermo, Bodo (Proicazekia) parva, Eutosi])]ion. 

 sulcata 1)1, PIcuromonas jaculans. 

 Infusoria. — Lacrymaria olor, CycJidiuni glaucoma. 

 Glen (iii). This was a sample of soil from virgin grass veld, 

 giving a very feebly acid reaction to litmus. No organisms were 

 observed on direct examination, and in water cultures very few 

 Protozoa appeared. These were: — 



Rhizopoda. — Aniceba verrucosa, Euglypha sp. 

 Mastigophora. ■ — Peranema trichophoruni , Olhonionas 



ternio, Bodo (P.) parva. 

 Infusoria.- — Colpoda cucullus, CycJidiuni glaucoma. 

 Glen (iv). This sample was collected from a cultivated 

 experimental plot of grass veld used for depth ploughing experi- 

 ments. The soil was a sandy loam overlying ironstone, and was 

 neutral to litmus. No organisms were detected on direct exami- 

 nation. Water cultures were made and the following organisms 

 were obtained : — 



Rhizopoda. — Amoeba proteus, A. guttula, Euglypha alrco- 



lata, Euglypha sp. 

 Mastigophora. — Peranema trichophoruni , Oikonionas iermo, 



Bodo (P.) parva, Pleuromonas jaculans. 

 Infusoria. — Paramoe.cium aurelia, Cyclidin ni glaucoma, 

 Euplofes harpa. 

 Glen (v). This sample was collected during bright sunshine 

 froin an uncultivated, infertile patch of soil, 200 yards from the 

 Zoetewoud boundary line. It was a crumbly light red loam, 

 apparently brak, and was acid to litmus. No Protozoa were 

 detected on direct examination. In water cultiu-es the following 

 Protozoa were seen : — 



Rhizopoda. — Amoeba Umax, A. guttula, Euglypha sp. 

 Mastigophora. — Peraneyna trichophorum, Oihomonas termo, 



Bodo (P.) parva, Herpetomonas terricola-. 

 Infusoria. — Cyclidium glaMcoiua, Halieria. grandinclla. 

 Glen (vi). This sample of soil was obtained from an infertile 

 patch of ground that had been cultivated under mealies for about 

 two seasons. The sample was a mixed one of the top six inches 

 of soil. Its reaction to litmus was feebly acid. No Protozoa 

 were seen on direct examination. The following Protozoa 

 were obtained in water cultures in very small numbers: — 

 Rhizopoda. — Amoeba guttula, Euglypha sp. 

 INIastigophora. — Peranema trichophorum , Oikomonas termo, 

 Bodo (P.) parva, Pleuromonas jaculans, Herpeto- 

 monas terricol(p. 

 Infusoria. — Lionotvs fasciola, (' nclidiiim glaucoma . 

 Negative periods of several days' duration occurred, when no 

 Protozoa were detected in the culture. 



