SOIL PROTOZOA IX SOUTH AFRICA. 353 



proved rich in Protozoa subsequently. The following motile 

 Protozoa were observed: — 



Ehizopoda. — A))io^ba pvofcun, A. liinax, A. verrucosa, A. 

 guttula, A. radiusa, Diffugia globnlosa, Euglypha 

 alvcolata, EuglypJia sp. 

 Heliozoa . — Actinophrys sol. 



Mastigophora. — Peranema iricJioplioriDn . Oi]:o)nunas tcrino, 



Bodo (P.) parva, Euglena viridis, E. oxyuris, Enio- 



siphon sulcatum, a Herpetomonas on one occasion. 



Infusoria. — Holophrya ovum, Lacrymaria olor, Amphileptus 



cygnus, Coleps hirtus, (Jolpoda cucullus, CycUdiuin. 



glaucoma, Spirostomiun ambiguum, Sfyloiiychia 



miitilu.s, Euplotes harpa, Uroleptus piscis. 



In addition to the foregoing Protozoa, many of which are 



normal occupants of soil and fresh water, certain encysted 



Protozoa, some pathogenic, some harmless to man, were also 



found. These included tetranucleate cysts of Entamoeba 



liistolytica, cysts of Entamoeba coll containing eight nuclei, and 



typical oval cysts of Giardia (Laniblia) i)itcsti)ialis. As the 



methods for disposal of excrement by natives and others are 



somewhat primitive, and as night soil is used as fertiliser, this 



result is not altogether unexpected. Cultures 'of this soil also 



revealed ova of Trematodes and Nematodes and some free-living 



small Nematodes. 



Botanic Gardens, Lourengo Marques. — This specimen was 

 collected during tine weather on July 14, from the side of a 

 freshly cut trench that was slightly shaded by bamboos. It was 

 a dark brown, sand}- soil, containing a slight admixture of bamboo 

 roots, and was a mixed sample of soil from the surface to six 

 inches deep. Its reaction to litmvis was feebly acid. 



A culture in the proportion of 5 grams of soil to 10 c.c. of 

 boiled sterile water was made. It showed no motile Protozoa 

 when examined immediately after being made. The following 

 organisms were found subsequently, but never in large numbers : 

 Ehizopoda. — Amoeba proteus, Euglypha sp. 

 Mastigophora. — Oikomonas termo, Bodo (P.) parva. 

 Infusoria. — Lacrymaria olor, Colpoda cucullus, Aspidisca 

 costata, Stylonychia mytilus, Uroleptus piscis, 

 Euplotes harpa . 

 A few bacteria were occasionally observed in this culture. 



Marracuene. — This sample was collected on July 16. It 

 was a mixed sample of soil from the surface to three inches deep, 

 light brown, sandy, and was obtained from an uncultivated agave 

 plantation. Its reaction to litmus was very feebly acid. A 

 culture in the pro]5ortion of 5 grams of soil to 10 c.c. of boiled 

 sterile water yielded very few Protozoa. These were: — 



Rhizopoda. — Amoeba Umax, Euglypha sp. 



Heliozoa. — Actinophrys so] . 



Mastigophora. — Oikomonas tcrmo, Bodo (P.) parva. 

 An eight-nucleate cyst resembling that of Entamoeba coli 

 was also observed. Ciliates wei*e not found in this soil culture, 



