354 SOIL I'ltOTOZOA IX SOUTH AFIMCA. 



though relatively large numbers of genera of ciliates occurred in 

 the other samples of soil from Portuguese East Africa. 



The three soils showed considerable difterences of fauna 

 among themselves, but practically all the genera and species of 

 Protozoa found therein occur also in soils that we have examined 

 from the Union of South Africa. 



Kesults of Examinations of Watkulogged Soils. 



Samples of waterlogged soils were obtained from five 

 localities in the neighbourhood of Johannesburg, and were 

 examined for the presence of trophic Protozoa. The method of 

 examination was the same as previously detailed on page 385 of 

 our paper of last year. 



Bedfcrd Court, near Johannesburg. — Two samples of soil 



wer',' obtaiiu'd on April 24 during the dry season. 



(i) Waterlogged soil from the edge of the open-air swimming 

 bath was taken at the level of the water. The soil was black, 

 mixed with some sand and vegetable debris, and gave an acid 

 reaction to litmus. 



One-fifth c.c. of ooze contained the following Protozoa: — 

 Rhizopoda. — 1 Diffiugia globiilosa. 

 Mastigophora. — 3 Bodo {P.) parva. 



Infusoria. — 3 Coleps hirtiis, 1 P(ii-a)}ioeciit))i putri)tu))i, 2 

 iHtylonychia mytilus. 



It may be of interest to add that a water culture of this soil 

 was also made for comparison. The same Protozoa as were 

 recorded from direct examination occurred in the culture, but in 

 addition Amoehcu Umax, Plcuroinonas jacula)i.s and CycluJni ni 

 (jiaucoDia were also seen. 



(ii) Sample of soil from the edge of the swimming bath, 

 taken three inches below the surface of the water, was examined. 

 The soil was black, not sandy, contained vegetable debris and 

 was foul smelling. Two samples of the ooze were examined. 



(a) One-fifth c.c. of ooze contained: 



Infusoria. — 1 Pnranta'c'uim putrliniin , 1 SfjiJonychia mytilus 



(b) One-fifth c.c. of ooze contained one P(ir(i))ia'cmm 

 putrinum only. 



A water culture of this soil showed, in additioi;, after 22 days, 

 one Paramcrciiii)} aureJia and one A)no'ha lii)ni.r. 



Mulder's Drift. — A sample of soil was obtained from the 

 bank of the drift in fine weather on September 14. It was 

 black waterlogged soil giving a weak acid reaction to litmus. 

 One-half c.c. of ooze contained the following Protozoa: — 



Mastigophora. — 2 Euglena viridis, 1 Englena oxyuris. 

 Infusoria. — 3 CnJcps hirfu!^, 1 Colpoda curuUus, 8Cyclidium 

 g]auco))ui. 



Florida. — A small sample of waterlogged soil was obtained 

 from the banks of Florida Lake on September 19. The soil gave 

 an acid reaction to litmus. One-fifth c.c. of ooze yielded some 

 interesting tropliic Protozoa nf)t hitherto recorded in direct 



