SOIL PROTOZOA IX SOUTH AFRICA. 355 



examinations of soil from other localities in South Africa. The 

 following organisms were observed: — 



Mastigophora. — 2 Euglena oxyuris, 1 Petalomonas pleuro- 



fiig)na, 1 Entosiphon sulcatum. 

 Infusoria. — 1 LoxopJiijllum rostratum, 2 Colpoda cucuUus, 

 8 Faramceciuui putrinum. 

 The occurrence of Petalomonas pleurosigma and Loxophyllum 

 rostratum is of much interest. Entosiphon sulcatum has been 

 found in cultures of soils from other localities, but has not been 

 detected in such soils by direct examinations. 



Canada Junction, near Johannesburg. — One sample of water- 

 logged soil was obtained at the commencement of the Transvaal 

 rainy season on November 6, after three days' heavy rain. This 

 soil had been under water for three days. The sample was a 

 mixed one of the first three inches of soil, from a spot ten feet 

 from a farm dam, where normally the soil was always slightly 

 moist. 



The soil was a black loam, with some sand and much plant 

 debris. It gave an acid reaction to litmus. Samples of one-half 

 c.c. of ooze from this soil were examined at intervals of four and 

 eight hours after the sample had been collected. The following 

 Protozoa in the trophic condition were observed : — 



Rhizopoda. — 1 Amoeba verrucosa, 1 Euglypha sp. 



Mastigophora. — 2 Oikomonas tcrino, 2 Bodo (P.) parva. 



Infusoria. — 3 Lacrymaria olor, 1 Cyclidium glaucoma. 

 Two cysts of Antoeba verrucosa were also found, in addition 

 to the trophic motile specimen. Practically no difference was 

 noted in the ooze examined at four hours and at eight hours. 



Milner Park, Johannesburg. — This sample was collected on 

 November 8 from the side of a donga in which three inches or 

 more of rain had lain for a week. The fluid that oozed from the 

 feebly acid, brownish red sandy loam was examined, and the 

 following Protozoa were found in one-half c.c. of the ooze: — 



Mastigophora. — 5 Bodo (P.) parva, 2 Oikomonas termo, 



1 Pe ran em a trichophorum. 

 Infusoria. — 2 Colpoda cucullus, 1 Paramoecium aurelia. 



Geographical Distribution* of Protozoa as Ascertained by 

 Cultures of Nox-Waterlogged Soils. 



The geographical distribution of Protozoa in South African 

 soils recorded by us last year (this Jourxal, vol. XVIII, pp. 382- 

 385) can now be amplified and extended by the addition of other 

 localities and the inclusion of organisms not listed heretofore. 

 Ehizopoda. 



Ama'ba j)roteus, found in soils from Gwaayang (George 

 district), Jonkersberg (good and poor pine lands), 

 Potchefstroom, Onderstepoort, Glen (locality iv), 

 Bloemfontein (cultivated and uncultivated soils), 

 Lourenco Marques (Observatory and Botanic Gardens). 

 Amceba Umax, found in soils from Piosebank, Gwaayang, 

 Onderstepoort, Winburg (cultivated soil), Glen 



