Blood Parasites. 2H3 



parasite, whirli is oval, is more hyaline, the nucleus very 

 diffuse, and the melanin pigment granules disposed at either 

 pole. 



The fully-developed female gametocytes (Figs. 3, 9 and 1-'^) show 

 a spherical outline, a more granular body, a more or less general 

 distribution of the large melanin granules and a more definite 

 clear nucleus, except in Fig. 3, in which no nucleus could be 

 detected at all. No erythrocytes could be found in which the 

 parasite had occasioned the loss of the host nucleus ; nor were 

 any free forms to be found except that in Fig. 10, in which the 

 body was hyaline with a faintly staining diffuse nucleus near 

 the broader end, and the partly free much more coarsely 

 granular form in Fig. 5. Five typical forms may be described 

 as follows : — 



1. Ihe nucleus of the red blood corpuscle is pushed close to 

 the edge, there being little cytoplasm in the host-cell, the 

 greater portion of which is occupied by the parasite. The 

 latter has a very slightly eccentric homogeneous nucleus stain- 

 ing pink (with Giemsas stain), the finely granular protoplasm 

 staining a deep blue. The body contains irregular but evenly 

 distributed fine pigment granules. No limiting membrane was 

 present (cf. Fig. 9). 



2. The nucleus of the host-cell which contains a large amount 

 of protoplasm is pushed to one side. The parasite has a large 

 pink-stained nucleus almost filling the body with faint blue 

 protoplasm at either side, containing fine granules (cf. Fig. 15). 



3. Host-cell is not always enlarged, but the nucleus is pushed 

 to one side ; the parasite is very faint and pinkish ..through- 

 out. Large granules are distributed, more or less generally. No 

 distinct nucleus is to be seen. Definite limiting membrane is 

 present (cf. Fig. 4). 



In such as shown in Fig. 6, two vesicular nuclei are seen 

 within a very granular protoplasmic body. 



4. The host-cell is enlarged and somewhat i)yriform. The 

 nucleus is slightly displaced by the oval parasite, which has a 

 diffuse pinkish sometimes eccentric nucleus, in a bluish body, 

 more deeply stained at one extremity. The pigment granules 

 are irregular, but more or less polar in distribution (cf. Fig. 

 H). 



