.'!2 Georgina Stveet : 



worm-tunnels have a considerable quantity of fibrous tissue. These 

 nodules are inseparable except by cutting from the skin, as con- 

 trasted with the smooth surfaced compact capsule of 0. gibsoni in 

 either Bos taunt* or Bos indicus, or of 0. indica in Bos indicus. 

 This firm attachment to the skin in the buffalo is attributed by 

 Mr. Wilson "to the density of the subcutal structures in that 

 animal." He also states that "the ' nests' show more tendency to 

 calcify quickly in the buffalo than in cattle," but that he has 

 " failed to notice any variation in colour " such as was mentioned 

 by one observer. The hairlessness of the skin over the nodule, noted 

 by the same observer, is apparent slightly, in one of the specimens, 

 but I cannot detect any thinning of the skin in that position. These 

 nodules were taken from the vicinity of the sternum in three 

 buffaloes, 8, 11 and 12 years old respectively, considerable caseation 

 being present in several, e.g., in II., in which the head and tail of 

 the female were not found, and III., in which the head of the male 

 is missing. (See Tables 9, 10 and 11.) Nodule II. had two males 

 with the one female, while one male only was present in each of the 

 others. Commencing caseation also accounts for some uncertainty 

 in regard to the anal papillae in the males II. 1 and II. 2, while 

 the tail of the male III. was much distorted, the long spicule having 

 torn through the postanal tissues. 



Reference to the tables (9, 10, and 11) herewith added, and their 

 comparison with those previously given will be found to establish 

 the conclusion that these nodules in Bos bubal is in India are 

 caused by the same species of worm as causes those in Bos indicus 

 in India, viz., 0. indica. In the male the thicker anterior end, 

 the longer thicker oesophagus, the slightly more anterior cloacal 

 opening, the thick and longer large spicule characteristic of 0. 

 indica are all present, while in I., and as far as can be seen in II. I 

 and II. 2, the anal papillae are such as are found in that species. 

 In the female, the thinner anterior end characteristic of 0. 

 indica is found, with a fairly long oesophagus, and also a more 

 posteriorly placed anus, but the other features are not so character- 

 istic, though still such as are found in other specimens of 0. indica. 



It will be seen that this further material entirely confirms the 

 conclusions arrived at in the body of this Report (1) that the 

 nodule worm of India is a new species different from that of Aus- 

 tralia, (2) that the nodule worm of Siam or Malaya is similar to 

 that of Australia, (3) that the Indian buffalo has not been the carrier 

 of this parasite into Australia, and (4) that Malaya or the Malay 

 Archipelago has been the source of the infection of Australian cattle. 



