Hi I Gwynneth Buchanan : 



of many basophil granules. In others (Gram, barbata) the proto- 

 plasm tended to be vacuolated, and in Til. scincoides this vacuo- 

 lation extended to the nucleus (cf. Erhlich, p. 86). As in amphibia 

 Cnuier regards these cells as playing the part of macrophages, true 

 polymorphs being absent. 



Polymorphonucleate Cells. — These were roughly about the same- 

 size as the eosinophiles, but gave a small percentage count except 

 in young Gramat. muricata, where they ran up to 42 per cent, of 

 total leucocytes; while in the Monitor it was not possible to dis- 

 tinguish them from the eosinophil cells. In smears from Chel. 

 longicollis, large cells were seen in which the nucleus was pressed 

 to one side, and the protoplasm scarcely stained at all. In other 

 cases, e.g., Gram, barbata forms containing a horseshoe-shaped 

 nucleus,, and faintly pink protoplasm, scarcely to be distinguished 

 from <'osinophils, and comparable to those of some frogs, were seen. 

 Similar cells were noted in Til. nigra-lutea. In other cases, e.g. 

 Gram, muricata. the protoplasm was vacuolated, and the nucleus 

 not so strongly basophil, as in the ordinary types. These, under 

 Gruner's classification, must be regarded as eosinophils 



Eosinophil Cells. — These were fairly well marked, though giving 

 small percentage counts, except in Chel. longicoUis, in which the 

 L-ra miles could be distinctly divided into small and large. The 

 latter closely resembled the spindle-shaped structures of birds, and 

 were much more numerous than the former, the total eosinophil 

 count giving 40 per cent, of leucocytes present. In size, with the 

 exception of Chel. longicoUis, which ran as high as 21.5m, these- 

 cells average about the same as the polymorph forms. The 

 eosinophils of Chel. longivollis are further peculiar in showing, 

 besides the spindle-shaped granules already mentioned, finely and 

 coarsely granular cells, the latter not unlike those of Lymnody- 

 nas.tes dorsalis, among the amphibia, though not staining so dis- 

 tinctly. In most cases, also, in this species, the eosinophil granules.. 

 with the exception of the large ones, did not stain with Jenner 

 at all. so making it hard to distinguish true polymorphs from 

 eosinophils, both of which carry nuclei pressed to the side. The 

 -■iimc fact was also observed in Gram, barbata. In Til. nigra-lutea 

 these cells did not show- up with either Jenner or Giemsa; while 

 Trach. rugosus, both young and adult, showed scattered granules 

 staining hest with Jenner, some cells being not clearly granular, 

 while all had the nucleus pressed to one side. On the other hand, 

 in Til. scincoides the nucleus was round iii distinction to that of 

 the polymorph cells, and the granules were few and refractive. 

 Gruner questions the analogy between the cells containing spindle- 



